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eEMLP02

Plasmid

#238379

Purpose

TurboRFP part plasmid for Golden Gate Assembly

Depositor

Richard Murray

Publication

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

TurboRFP

Expression

Bacterial

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRD4

Plasmid

#183331

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRD4 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRD4

Use

Lentiviral

Available since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_aMFx1 (4a)

Plasmid

#238900

Purpose

Plasmid insert contains the aMFx1 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_aMFx1

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_aMFx1_noKREAEA (4a)

Plasmid

#238901

Purpose

Plasmid insert contains the aMFx1 noKREAEA C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_aMFx1_noKREAEA

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_aMFx4 (4a)

Plasmid

#238902

Purpose

Plasmid insert contains the aMFx4 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_aMFx4

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_TEV_aMFx1 (4a)

Plasmid

#238903

Purpose

Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_TEV_aMFx1

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_FLAG_aMFx1 (4a)

Plasmid

#238904

Purpose

Plasmid insert contains the aMFx1, FLAG and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_FLAG_aMFx1

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_HA_aMFx1 (4a)

Plasmid

#238905

Purpose

Plasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_HA_aMFx1

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_aMFx1 (4a)

Plasmid

#238906

Purpose

Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_aMFx1

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Vector

Plasmid

#239611

Purpose

Transposon vector

Depositor

Xin-yuan Guan

Publication

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB033

Plasmid

#239387

Purpose

Super folding Green Fluorescence Protein (sfGFP)

Depositor

Xiaohan Yang

Publication

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

Superfolder GFP (gfp )

Use

Synthetic Biology

Expression

Plant

Promoter

35S

Available since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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p362_DG

Plasmid

#234684

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-Opsin

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His6-3C-3XFLAG-SUMO and Thrombin-Opsin

Expression

Bacterial

Mutation

No

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1052

Plasmid

#209542

Purpose

BTK Type 2 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Publication

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 2 Kanamycin resistance cassette

Use

Synthetic Biology

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pZB641_pT7_Lysin-KU1

Plasmid

#228511

Purpose

T7 RNAP inducible Lysin KU1 Expression

Depositor

Timothy Whitehead

Publication

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

Lysin KU1

Use

Synthetic Biology

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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OgeuIscB ωRNA-v2

Plasmid

#220958

Purpose

human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

Depositor

Weixin Tang

Publication

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning

Use

CRISPR

Expression

Mammalian

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pK2-J11

Plasmid

#218224

Purpose

Terminates AtU6-promoter driven sgRNA array with two guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK4-J11

Plasmid

#218225

Purpose

Terminates AtU6-promoter driven sgRNA array with four guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK6-J11

Plasmid

#218226

Purpose

Terminates AtU6-promoter driven sgRNA array with tsix guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK8-J11

Plasmid

#218227

Purpose

Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible

Depositor

Daniel Voytas

Insert

linker sequence

Use

CRISPR

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMBA325

Plasmid

#214742

Purpose

Medium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Publication

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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