We narrowed to 12,028 results for: LEA

Showing: 9901 - 9920 of 12028 results

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pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

Plasmid

#188274

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX(T26R/K610R/K808R)

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-C-TEV-HAT

Plasmid

#202597

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_367-496

Plasmid

#196530

Purpose

Entry vector for Gateway with Eps8L2_367-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_1-183

Plasmid

#196527

Purpose

Entry vector for Gateway with Eps8L2_1-183

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-183 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_544-715

Plasmid

#196532

Purpose

Entry vector for Gateway with Eps8L2_544-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_181-299

Plasmid

#196528

Purpose

Entry vector for Gateway with Eps8L2_181-299

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGST-parallell2-GGA3(571-723)

Plasmid

#199150

Purpose

Expresses GST-GGA3(571-723) in bacteria

Depositor

Juan Bonifacino

Article

Miller et al Nat Struct Biol. 2003 Aug;10(8):599-606.

Insert

GGA3(571-723) (GGA3 Human)

Tags

GST and TEV protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-FHIP-L

Plasmid

#198528

Purpose

Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

FHIP-L (FHIP1A Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

Plasmid

#197970

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

C328S, C331S, L1332V, delta(1616-1666)

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot8_R

Plasmid

#147370

Purpose

Bacterial Expression of HsNot8

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot8 (CNOT8 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot4_F

Plasmid

#146275

Purpose

Insect Expression of DmNot4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot4 (CG31716 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmNot4-EGFP_F

Plasmid

#146273

Purpose

Insect Expression of DmNot4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot4 (CG31716 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_529-715

Plasmid

#196533

Purpose

Entry vector for Gateway with Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Plasmid

#186703

Purpose

Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dMET

Plasmid

#176031

Purpose

A knock-out vector for dog MET

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 exon

Plasmid

#176033

Purpose

A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 intron

Plasmid

#176224

Purpose

A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FnRPR9-10

Plasmid

#182568

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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FnRPR8-10

Plasmid

#182570

Purpose

Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 8-10

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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