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We narrowed to 9,922 results for: pET

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Showing: 9901 - 9920 of 9922 results

  1. pJ139

    Plasmid
    #29489
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  2. pJ138

    Plasmid
    #29488
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  3. PBCV-1 Lig-N-HiBiT

    Plasmid
    #215439
    Purpose
    Bacterially expressed PBCV Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Paramecium bursaria Chlorella virus 1 ATP-dependent DNA ligase, N-HiBiT tag, N-10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215439-plasmid-map-sequence-id-428715

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  4. PBCV-1 Lig-C-HiBiT

    Plasmid
    #215440
    Purpose
    Bacterially expressed PBCV Lig-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Paramecium bursaria Chlorella virus 1 ATP-dependent DNA ligase, C-HiBiT tag, C-10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215440-plasmid-map-sequence-id-428722

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  7. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid
    #118269
    Purpose
    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure
    Depositor
    Douglas Rees
    Publication
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…
    Promoter
    T7 Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118269-plasmid-map-sequence-id-228542

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  8. pJ143

    Plasmid
    #29490
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. His6-SUMO-SPMc-SpyCatcher003-Ctag

    Plasmid
    #246648
    Purpose
    Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligation
    Depositor
    Mark Howarth
    Publication
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    His6-SUMO-SPMc-SpyCatcher003-Ctag
    Tags
    Ctag, His-tag, SUMO, and SpyCatcher003
    Expression
    Bacterial
    Mutation
    The SPMc comprises the C-terminal fragment of th…
    Promoter
    T7
    Available since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246648-plasmid-map-sequence-id-491284

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  13. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid
    #173762
    Purpose
    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Publication
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173762-plasmid-map-sequence-id-353460

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  14. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid
    #173763
    Purpose
    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Publication
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173763-plasmid-map-sequence-id-353459

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  15. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid
    #173764
    Purpose
    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Publication
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173764-plasmid-map-sequence-id-353458

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  16. ybbR-ddfln4-RLC-ELP-LA-Xmod-DocIII-Ctag

    Plasmid
    #257336
    Purpose
    Polyprotein for applying force to the N-terminus of the RLC and the C-terminus of the lever arm of the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Publication
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120, TEV, Xmod, ddfln4, an…
    Expression
    Bacterial
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257336-plasmid-map-sequence-id-512392

    Enlarge

  17. AviTag-His6-OAZ-SPM(FrpA)-Ctag

    Plasmid
    #246646
    Purpose
    Expresses AviTag-His6-OAZ-SPM(FrpA)-Ctag in bacterial cells for calcium-induced NeissLock protein ligation
    Depositor
    Mark Howarth
    Publication
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    AviTag-His6-OAZ-SPM(FrpA)-Ctag
    Tags
    AviTag, Ctag, and His-tag
    Expression
    Bacterial
    Mutation
    Residues 95-219 of human Orinithine Decarboxylase…
    Promoter
    T7
    Available since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    246646-plasmid-map-sequence-id-499057

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  18. RLC-ELP-ybbR-LA-GB1-GB1-Xmod-DocIII-Ctag

    Plasmid
    #257334
    Purpose
    Polyprotein for applying force to the N- and C-termini of the lever arm in the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Publication
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120 (Elastin-Like Polypept…
    Expression
    Bacterial
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257334-plasmid-map-sequence-id-512397

    Enlarge

  19. LA-ELP-ybbR-RLC-ddfln4-Xmod-DocIII-Ctag

    Plasmid
    #257335
    Purpose
    Polyprotein for applying force to the N- and C-termini of the RLC in the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Publication
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120 (Elastin-Like Polypept…
    Expression
    Mammalian
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257335-plasmid-map-sequence-id-515911

    Enlarge

  20. ΔN_RLC-ELP-ybbR-LA-GB1-GB1-Xmod-DocIII-Ctag

    Plasmid
    #257337
    Purpose
    Polyprotein for applying force to the N- and C-termini of the lever arm in the RLC complex of human β-cardiac myosin, where residues of 2-22 of the RLC were deleted.
    Depositor
    Tom Perkins
    Publication
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120, GB1, TEV (TEV cleavag…
    Expression
    Bacterial
    Mutation
    Amino acids 2 to 22 are deleted and only amino ac…
    Promoter
    T7
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257337-plasmid-map-sequence-id-512398

    Enlarge

Showing: 9901 - 9920 of 9922 results