We narrowed to 15,941 results for: nans

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MCS-3_Endofin_LgBiT

Plasmid

#182574

Purpose

This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

Depositor

Bradley McConnell

Article

Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

Insert

zinc finger FYVE-type (ZFYVE16 Human)

Tags

LgBiT (Large BiT)

Expression

Mammalian

Mutation

FYVE domain Q739-K806

Promoter

Herpes simplex virus (HSV)

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-mCherry

Plasmid

#137132

Purpose

Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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nLuc-GLP-1R-His-FLAG

Plasmid

#124831

Purpose

Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

Depositor

Sam Gellman

Article

Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

Insert

GLP1R (GLP1R Synthetic, Human)

Use

Luciferase

Tags

FLAG, His6, and NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9-Geminin

Plasmid

#109401

Purpose

Expresses Cas9 fusion to Geminin degron domain

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

Geminin (GMNN Human)

Mutation

Geminin degron fragment

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-zDjCas13d-His

Plasmid

#234481

Purpose

Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DjCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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PJ-DEAD

Plasmid

#38002

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1 (SAC1 Budding Yeast)
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Mutation

Asp 1263 mutated to Ala and Cys 779 mutated to Ser

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL5A-COMP5AP-AviTag-9xHis

Plasmid

#157207

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5A (KIR2DL5A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA5A-COMP5AP-AviTag-9xHis

Plasmid

#157572

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA5A (SEMA5A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-MLH1dn (mouse) for IVT

Plasmid

#246485

Purpose

Template for in vitro transcription of mouse MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (Mlh1 Mouse)

Use

Template for in vitro transcription

Mutation

deletion of residues 758-760

Promoter

T7

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-Tau2N4R-WPRE

Plasmid

#226380

Purpose

Expresses tau propagation ORF with no V5 tag on WT 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

TagRFP.T-2A

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-rsChRmine-oScarlet-WPRE

Plasmid

#183528

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet

Use

AAV

Mutation

I146M/G174S

Promoter

Ef1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-ProDOL

Plasmid

#206866

Purpose

Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag

Depositor

Dirk-Peter Herten

Article

Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

Insert

ProDOL

Use

Retroviral

Tags

HaloTag, His-Tag, SNAP-tag, and eGFP

Expression

Mammalian

Mutation

Contains Lyn amino acids 1-16

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis

Plasmid

#157142

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PILRA (PILRA Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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DOP1B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176979

Purpose

Mammalian expression vector encoding DOP1B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DOP1B (DOPEY2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Plasmid

#176534

Purpose

Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associated IscB (Delaware Bay metagenome)

Tags

14xHis-MBP-bdSUMO and TwinStrep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMIG-IRF4

Plasmid

#58987

Purpose

MSCV based expression vector - co-expresses GFP and murine IRF4

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Insert

Mouse IRF4 (Irf4 Mouse)

Use

Retroviral

Tags

Kozak sequence added

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL5B-COMP5AP-AviTag-9xHis

Plasmid

#157208

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5B (KIR2DL5B Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

Plasmid

#55645

Purpose

Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV5, AAV8, and AAV9

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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GAG-CRErec

Plasmid

#119971

Purpose

Expresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG-nlsCRErec

Tags

nls

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT6_SEPT7-TEV-Strep

Plasmid

#174499

Purpose

bacterial co-expression of human SEPT6 and of human SEPT7

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN6 (SEPTIN6 Human)
SEPTIN7 (SEPTIN7 Human)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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