We narrowed to 10,047 results for: pET

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pAG415Gal_Gcn4 ILVtoED WHA

Plasmid

#232995

Purpose

Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAHA

Plasmid

#232997

Purpose

Galactose iduced expression of Gcn4 9acidAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidA

Plasmid

#232967

Purpose

Galactose iduced expression of Gcn4 9acidA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+GFP

Plasmid

#233002

Purpose

Galactose iduced expression of Gcn4 Aro3+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED WGFP

Plasmid

#233007

Purpose

Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED solvvol

Plasmid

#231858

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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E547* FL USP7

Plasmid

#229001

Purpose

E547-mutant of human USP7

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 1-546 (USP7 Human)

Tags

6xHis cleavable with thrombin

Expression

Bacterial

Mutation

E547 mutated to stop codon

Promoter

T7

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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G392A FL USP7

Plasmid

#227217

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

G392A

Promoter

T7-promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG8Ha-EWS_RRM(359-447)

Plasmid

#188045

Purpose

Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Publication

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-358, 446-656

Promoter

T7

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-EWS_RRM-RGG(359-513)

Plasmid

#188046

Purpose

Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Publication

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-358, 514-656

Promoter

T7

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pJB50

Plasmid

#226311

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-7

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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N418S FL USP7

Plasmid

#227215

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

N418S

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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G392D FL USP7

Plasmid

#227216

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

G392D

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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K420E FL USP7

Plasmid

#227223

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

K420E

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L373F FL USP7

Plasmid

#227220

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

L373F

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Y331H FL USP7

Plasmid

#227218

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

Y331H

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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P273H FL USP7

Plasmid

#227221

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

P273H

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M225I FL USP7

Plasmid

#227219

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

M225I

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L469R FL USP7

Plasmid

#227225

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

L469R

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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E345K FL USP7

Plasmid

#227224

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al Proc Natl Acad Sci U S A. 2025 Sep 30;12

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

E345K

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge