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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Publication

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MH6-RspWYL1

Plasmid

#108306

Purpose

Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2-IRES-GFP

Plasmid

#107146

Purpose

Mammalian expression of mouse Ezh2

Depositor

Martine Roussel

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT7-7 asyn A30P

Plasmid

#105726

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Publication

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A30P (SNCA Human)

Expression

Bacterial

Mutation

A30P

Promoter

T7

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PPARgamma2

Plasmid

#78768

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPARG (Pparg Mouse)

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
HIR K1030R

Plasmid

#24050

Depositor

Frederick Stanley

Publication

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

K1030R

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKD2.RNAi

Plasmid

#10816

Depositor

Alex Toker

Publication

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD2 RNAi (PRKD2 Human)

Use

RNAi

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
3534 pcDNA3 Bax

Plasmid

#8783

Depositor

Stanley Korsmeyer

Publication

Oltvai et al Cell 1993 Aug 27;74(4):609-19.

Insert

Bax (Bax Mouse)

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
p4055 HA-E6AP isoform III

Plasmid

#8659

Depositor

Peter Howley

Publication

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform III (UBE3A Human)

Tags

HA

Expression

Mammalian

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Publication

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Expression

Mammalian

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE unc13a GFP KI

Plasmid

#131498

Purpose

Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Unc13a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-ATF4

Plasmid

#125237

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Publication

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Tags

RFP

Expression

Mammalian

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO_EGFP-GFE3

Plasmid

#79871

Purpose

For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Publication

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Has service

AAV1

Insert

GFP-GFE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

EF1a

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YIP sHRPa-Aga1P

Plasmid

#73151

Purpose

Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-Aga1P

Tags

V5 tag

Expression

Yeast

Promoter

glyceraldehyde-3-phosphate dehydrogenase GPD prom…

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS-PesaRlux

Plasmid

#47640

Purpose

wt esaR promoter in pCS26

Depositor

Cynthia Collins

Publication

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

PesaR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PesaR

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pRR-GR-5Z

Plasmid

#23060

Depositor

Charles Miller

Publication

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

GR (NR3C1 Human)

Expression

Yeast

Available since

March 16, 2010

Availability

Academic Institutions and Nonprofits only
Cdc2

Plasmid

#1886

Depositor

Sander van den Heuvel

Publication

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
TNFa-GFP

Plasmid

#163660

Purpose

Expresses GFP tagged TNFa in mammalian cells

Depositor

Lei Lu

Publication

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

tumor necrosis factor (TNF Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp4-EGFP

Plasmid

#165109

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Publication

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Publication

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

none

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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