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Plasmid#18975DepositorInsertLarge tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, (Tyrosine 376 changed to Alanine) and…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY1 mutant
Plasmid#18973DepositorInsertLarge tumor suppressor kinase 1 PY-1 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, Tyrosine 376 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY2 mutant
Plasmid#18974DepositorInsertNM_004690 (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY2 mutant (Tyrosine 559 changed to Alanine)PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
human KANK1 Ankyrin Repeats pET151
Plasmid#164053PurposeExpresses human KANK1 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3458)
Plasmid#160638PurposepEGB3a1 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)
Plasmid#213728PurposeMammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.DepositorInsertbIV spectrin (repeats 13-15) (Sptbn4 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-bII spectrin (repeats 13-15)
Plasmid#213729PurposeMammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.DepositorInsertbII spectrin (repeats 13-15) (Spnb3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
human KANK2 Ankyrin Repeats pET151
Plasmid#164054PurposeExpresses human KANK2 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK3 Ankyrin Repeats pET151
Plasmid#164056PurposeExpresses mouse KANK3 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4 Ankyrin Repeats pET151
Plasmid#164057PurposeExpresses mouse KANK4 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3459)
Plasmid#160639PurposepEGB3a2 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSDT (JBEI-11535)
Plasmid#87934PurposeCo-expression in S.cerevisiae of 4CL5 and AtSDTDepositorUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)
Plasmid#155194PurposeExpresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminusDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTruncated sequence of THBS1 (expresses Amino Acid…PromoterCMVAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
human KANK2-A670V Ankyrin Repeats pET151
Plasmid#164055PurposeExpresses human KANK2 Ank domain - A670V mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4-Y801H Ankyrin Repeats pET151
Plasmid#164058PurposeExpresses mouse KANK4 Ank domain -Y801H mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP
Plasmid#122955PurposeLentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expressionDepositorInsertNOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
UseLentiviralTags6xhis tagExpressionMutationdeletion of EGF repeats from full length NOTCH2NLBPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMY-empty-MCS
Plasmid#163351PurposeThe empty pMY backbone with multiple cloning siteDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only