Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1471 - 1500 of 6032 results

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pTubulin-miRFP713

Plasmid

#197240

Purpose

Expresses the fusion protein of Tubulin-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-deltaPYD

Plasmid

#51535

Purpose

Express His6-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCZGY553

Plasmid

#110883

Purpose

Pmec-4 Gateway Destination vector for cloning for expression in C. elegans mechanosensory neurons

Depositor

Andrew Chisholm

Article

Ghosh-Roy et al J Neurosci. 2010 Mar 3;30(9):3175-83. do

Insert

Pmec-4

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET-mGinger1

Plasmid

#119784

Purpose

Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mGinger1

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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P16ΔS:S10-(MCS)-3xHA

Plasmid

#108183

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmKate1-N1

Plasmid

#31906

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

alpha-actinin LSS-mKate1

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pSico-shNKCC1

Plasmid

#83027

Purpose

expresses an shRNA against NKCC1

Depositor

Angelique Bordey

Article

Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

Insert

shNKCC1

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-GST

Plasmid

#51533

Purpose

Express GST-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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bTub-GFP.766A

Plasmid

#112695

Purpose

expressed GFP in testes, under bTub promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

bTub-GFP-SV40

Use

Tags

Expression

Insect

Mutation

Promoter

bTub

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY2729

Plasmid

#135096

Purpose

Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrIV

Depositor

Yishi Jin

Article

Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

Insert

Hygromycin resistance

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

rps-0

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1

Plasmid

#113943

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-CAR-GECO1

Plasmid

#113939

Purpose

Bacterial expression of red fluorescent Ca2+ sensor CAR-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

CAR-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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LZF1723: mI12b-EGFP

Plasmid

#104123

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mI12b

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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P16ΔS:(MCS)-3xHA-S11

Plasmid

#108185

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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XFIVP Smad3

Plasmid

#14826

Depositor

Rik Derynck

Article

Feng et al Genes Dev. 1998 Jul 15. 12(14):2153-63.

Insert

Smad3 (SMAD3 Human)

Use

Tags

VP16 activation domain

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pDM_noro_A1_lacZA

Plasmid

#118818

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A1) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQFw

Plasmid

#61311

Purpose

Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70

Plasmid

#46124

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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P16ΔS:(MCS)-S11

Plasmid

#108184

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_sheep_catalytic domain

Plasmid

#214331

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-18_lemur

Plasmid

#214332

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Caspase-4_lemur_catalytic domain

Plasmid

#214328

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_sheep

Plasmid

#214335

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rabbit

Plasmid

#214334

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_dog

Plasmid

#214336

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Caspase-4_rabbit_catalytic domain

Plasmid

#214330

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE-RA5.0

Plasmid

#208312

Purpose

Expresses ABE-RA5.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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