Addgene

We narrowed to 6,111 results for: siae

Showing: 1471 - 1500 of 6111 results

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Use

Tags

Expression

Yeast

Mutation

Promoter

pTPI1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-HFL1-8flag-LEU

Plasmid

#207035

Purpose

Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

8xFLAG

Expression

Yeast

Mutation

Promoter

pHFL1

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pNZ18-CAH

Plasmid

#203159

Purpose

Replicates in E. coli, S. cerevisiae, and A. laidlawii

Depositor

Bogumil Karas

Article

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4D636K-Kina

Plasmid

#202725

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Use

Tags

mCherry- AID

Expression

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTSK573

Plasmid

#190881

Purpose

For C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiae

Depositor

Gunjan Mehta, Tatiana Karpova

Article

Podh et al STAR Protoc. 2022 Dec 16;3(4):101900. do

Insert

HaloTag

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAF9D94 (pAF10C6)

Plasmid

#185848

Purpose

Expressing artificial trans-repressor in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>CYC8>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJL15

Plasmid

#174069

Purpose

Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

pGal1

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H-H1

Plasmid

#163584

Purpose

express Dcp2 FL-H1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-326; the first HLM (EDQLKSYAEEQ…

Promoter

DCP2 promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300 AAAA

Plasmid

#163579

Purpose

express Dcp2 300 AAAA at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domain (301-970); mutati…

Promoter

DCP2 promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300 ΔH1

Plasmid

#163578

Purpose

express Dcp2 300 ΔH1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domain (301-970); Lysine…

Promoter

DCP2 promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Killer Protein SP

Plasmid

#153498

Purpose

S. cerevisiae Killer Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Killer Protein SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Acid Phosphatase SP

Plasmid

#153494

Purpose

S. cerevisiae Acid Phosphatase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Acid Phosphatase SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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RCP83

Plasmid

#163466

Purpose

Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

Depositor

Frank Albert

Article

Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

Inserts

Promoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 S389,398 A

Plasmid

#129485

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 S389,398 A (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Mutation of serines 389 and 398 to alanine

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 S389,398 D

Plasmid

#129486

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 S389,398 D (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Mutation of serines 389 and 398 to Asp

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Met25 Glo3 ΔGRM-ΔC

Plasmid

#129487

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 ΔGRM ΔC (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔC

Plasmid

#112653

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔC

Use

Tags

Expression

Yeast

Mutation

R59K, Δ431-493 (deletion of C-terminal amphipathi…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔGRM-ΔC

Plasmid

#112654

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔGRM ΔC

Use

Tags

Expression

Yeast

Mutation

R59K, Δ376-493 (deletion of Glo3 motif & C-te…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K S389,398 A

Plasmid

#112656

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398A

Use

Tags

Expression

Yeast

Mutation

R59K, mutations (S389A, S398A) in Glo3 motif, non…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K S389,398 D

Plasmid

#112657

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398D

Use

Tags

Expression

Yeast

Mutation

R59K, mutations (S389D, S398D) in Glo3 motif, pho…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 ΔGRM-ΔC

Plasmid

#112659

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP-Glo3 ΔGRM ΔC

Use

Tags

sfGFP

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 S389,398 A

Plasmid

#112660

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP- Glo3 S389,398A

Use

Tags

sfGFP

Expression

Yeast

Mutation

mutations (S389A, S398A) in Glo3 motif, non-phosp…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 S389,398 D

Plasmid

#112661

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP-Glo3 S389,398D

Use

Tags

sfGFP

Expression

Yeast

Mutation

mutations (S389D, S398D) in Glo3 motif, phosphomi…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K L246D

Plasmid

#112648

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K L246D

Use

Tags

Expression

Yeast

Mutation

R54K & L246D (in ALPS motif)

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K AxxA

Plasmid

#112649

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K AxxA

Use

Tags

Expression

Yeast

Mutation

R54K & Alanine substitution of C-terminal δL …

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 Δ3xF AxxA

Plasmid

#112650

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 Δ3xF AxxA

Use

Tags

Expression

Yeast

Mutation

R54K, W304A, W307A, W310A, W349A, F353A

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSP569

Plasmid

#139499

Purpose

Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl STL1 100 - 600

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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