We narrowed to 8,438 results for: app

1. pJBL2154

   Plasmid

   #71204

   Purpose

   pT181 STAR Antisense Expression Plasmid

   Depositor

   Julius Lucks

   Article

   Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

   Insert

   pT181 STAR Antisense
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RCAS IkB alpha M

   Plasmid

   #12407

   Depositor

   Inder Verma

   Insert

   IkB alpha M (Nfkbia Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Dominant-negative mutant. Many signal transductio…

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pJK383

   Plasmid

   #72474

   Purpose

   Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)

   Depositor

   T. J. Kappock

   Insert

   citrate synthase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-17 to phenylalanine, tryptopha…

   Promoter

   recA

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEC619

   Plasmid

   #182955

   Purpose

   SYNZIP18-Cascade.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

   Insert

   SynZip18-Cascade
   

   Use

   Tags

   SynZip18

   Expression

   Bacterial

   Mutation

   SynZip18 fusion to CasA

   Promoter

   J23150

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK605

   Plasmid

   #72530

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK623

   Plasmid

   #72540

   Purpose

   Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK289

   Plasmid

   #71553

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes lysine-39 to alanine (please see deposito…

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK501

   Plasmid

   #72246

   Purpose

   Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gln206 to Arg and changes tryptophan-151 to pheny…

   Promoter

   T7 and endogenous

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK502

   Plasmid

   #71582

   Purpose

   Produces Acetobacter aceti 1023 putative SixA with C-terminal His6 tag (SixAH6)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

   Insert

   phosphohistidine phosphatase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK304

    Plasmid

    #72225

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes alanine-368 to aspartate

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK303

    Plasmid

    #72224

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes alanine-367 to aspartate

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK579

    Plasmid

    #72426

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites

    Depositor

    T. J. Kappock

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    4 silent mutations introduce unique internal SpeI…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK522

    Plasmid

    #72510

    Purpose

    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK600

    Plasmid

    #71605

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJBL2191

    Plasmid

    #71205

    Purpose

    pbuE STAR Antisense Expression Plasmid

    Depositor

    Julius Lucks

    Article

    Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

    Insert

    pbuE STAR Antisense
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJBL1864

    Plasmid

    #71210

    Purpose

    Anti-anti Sense Expression Plasmid

    Depositor

    Julius Lucks

    Article

    Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

    Insert

    Anti-anti Sense
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJEC779

    Plasmid

    #234348

    Purpose

    Modular RENDR, GFP output, targets synthetic target

    Depositor

    James Chappell

    Article

    Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

    Insert

    Modular RENDR, GFP output
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. LP580

    Plasmid

    #90205

    Purpose

    pCMV-myc with cDNA encoding HsTRAPPC13 full length

    Depositor

    Lotte Pedersen

    Article

    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

    Insert

    TRAPPC13 (TRAPPC13 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    full length protein, no mutations

    Promoter

    CMV

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. LP539

    Plasmid

    #90203

    Purpose

    pCMV-myc with cDNA encoding HsTRAPPC8 aa residues 1-700

    Depositor

    Lotte Pedersen

    Article

    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

    Insert

    TRAPPC8 (TRAPPC8 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    aa residues 1-700

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK372

    Plasmid

    #73597

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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