Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pWzyE-GST-distrepII

Plasmid

#196666

Purpose

Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

GST and TEV-cleavable di-strep tag II

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-strep-mClover3

Plasmid

#196668

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C39A-Myc

Plasmid

#201167

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A

Promoter

MESV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_4-302_S

Plasmid

#147536

Purpose

Mammalian Expression of HsNot1iso1_4-302

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_4-302 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1356-2370_S

Plasmid

#147539

Purpose

Mammalian Expression of HsNot1iso1_1356-2370

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1356-2370 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1147-1717-K1277A_R

Plasmid

#147431

Purpose

Insect Expression of DmNot1_1147-1717-K1277A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-1717-K1277A (Not1 Fly)

Expression

Insect

Mutation

2 non silent mutations compared to the sequence g…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1149-2505-dsRNAres_R

Plasmid

#147432

Purpose

Insect Expression of DmNot1_1149-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1149-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNOT1_1-1083_R

Plasmid

#147441

Purpose

Bacterial Expression of DmNot1_1-1083

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1083 (Not1 Fly)

Expression

Bacterial

Mutation

4 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_416-1148_R

Plasmid

#147418

Purpose

Insect Expression of DmNot1_416-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_416-1148 (Not1 Fly)

Expression

Insect

Mutation

3 silent and two non silent N605S, K759M mutation…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2_1-436_R

Plasmid

#147419

Purpose

Insect Expression of DmNot2_1-436

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_1-436 (Rga Fly)

Expression

Insect

Mutation

one silent mutation C267T compared to the ORF ext…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_168-552-D412AN414A_R

Plasmid

#147427

Purpose

Insect Expression of DmCCR4_168-552-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552-D412AN414A (twin Fly)

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-D412AN414A_R

Plasmid

#147428

Purpose

Insect Expression of DmCCR4-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-D412AN414A (twin Fly)

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_168-552_I

Plasmid

#146612

Purpose

Insect Expression of DmCCR4_168-552

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552 (twin Fly)

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1-1148_I

Plasmid

#146613

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Expression

Insect

Mutation

four silent and two non silent N605S, K759M mutat…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1147-1717_I

Plasmid

#146614

Purpose

Insect Expression of DmNot1_1147-1717

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-1717 (Not1 Fly)

Expression

Insect

Mutation

2 non silent mutations compared to the sequence g…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1147-2505_I

Plasmid

#146615

Purpose

Insect Expression of DmNot1_1147-2505

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-2505 (Not1 Fly)

Expression

Insect

Mutation

4 silent and one non silent mutation G1999S compa…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2_1-401_I

Plasmid

#146616

Purpose

Insect Expression of DmNot2_1-401

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_1-401 (Rga Fly)

Expression

Insect

Mutation

one silent mutation C267T compared to the ORF ext…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3_1-246_I

Plasmid

#146618

Purpose

Insect Expression of DmNot3_1-246

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot3_1-246 (Not3 Fly)

Expression

Insect

Mutation

one silent and two non silent mutations compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3_239-681_I

Plasmid

#146619

Purpose

Insect Expression of DmNot3_239-681

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot3_239-681 (Not3 Fly)

Expression

Insect

Mutation

one non silent mutations compared to the sequence…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCR4_168-552_I

Plasmid

#146603

Purpose

Insect Expression of DmCCR4_168-552

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552 (twin Fly)

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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