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We narrowed to 1,118 results for: gibson assembly

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Showing: 981 - 1000 of 1118 results

  1. pmScarlet3-H_I3

    Plasmid
    #239343
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Publication
    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    I3-01
    Tags
    mScarlet3-H (a.k.a. mYongHong)
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239343-plasmid-map-sequence-id-474361

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  3. pZH518

    Plasmid
    #102671
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Publication
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102671-plasmid-map-sequence-id-199394

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  5. deltaVP1 + VP2C-GFP

    Plasmid
    #166676
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166676-plasmid-map-sequence-id-329830

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  7. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid
    #181790
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181790-plasmid-map-sequence-id-354811

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  9. pHS1625

    Plasmid
    #239834
    Purpose
    OrufIscB-REC E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-REC
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1
    Promoter
    T7
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    239834-plasmid-map-sequence-id-479361

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  12. pICH47732:FCP:ShBle

    Plasmid
    #85985
    Purpose
    Expresses ShBle with FCP promoter/terminator for F. cylindrus transformation.
    Depositor
    Thomas Mock
    Insert
    ShBle
    Use
    Golden gate assembly
    Promoter
    FCP
    Available since
    Jan. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    85985-plasmid-map-sequence-id-169710

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  17. pFP.R373

    Plasmid
    #138243
    Purpose
    Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GKA).SaP-dpol*.(CEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    July 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    138243-plasmid-map-sequence-id-304854

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  18. pFP.R366

    Plasmid
    #138236
    Purpose
    Expresses the split mCherry reporter interrupted by the LLP-Pol intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(AKA).LLP-Pol*.(CNF)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138236-plasmid-map-sequence-id-273512

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  19. pFP.R367

    Plasmid
    #138237
    Purpose
    Expresses the split mCherry reporter interrupted by the LP-Hel intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(ATG).LP-Hel*.(CGK)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138237-plasmid-map-sequence-id-273516

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  20. pFP.R356

    Plasmid
    #138226
    Purpose
    Expresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(DKF).Cro-RPB2*.(STK)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138226-plasmid-map-sequence-id-273504

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Showing: 981 - 1000 of 1118 results