We narrowed to 1,104 results for: gibson assembly
-
Plasmid#229995PurposePiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)DepositorTypeEmpty backboneUseCRISPRTagsNotI site for NEBuilder HiFi DNA Assembly with ss…ExpressionMammalianPromoterCAGAvailable SinceFeb. 28, 2025AvailabilityAcademic Institutions and Nonprofits only
-
pICH47732:FCP:ShBle
Plasmid#85985PurposeExpresses ShBle with FCP promoter/terminator for F. cylindrus transformation.DepositorInsertShBle
UseGolden gate assemblyPromoterFCPAvailable SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
PsaA
Plasmid#159997PurposeExpresses Streptococcus pneumoniae PsaA (19-309) in bacterial cellsDepositorInsertPsaA (19-309)
TagsHis6-tag and TEV cleavage siteExpressionBacterialPromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Spy0469
Plasmid#159996PurposeExpresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cellsDepositorInsertSpy0469 (24-389)
TagsHis6-tag and TEV cleavage siteExpressionBacterialPromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV mAG h-Geminin
Plasmid#206281PurposeENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMVDepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 CMV mKO2-hCdt1
Plasmid#206282PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMVDepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R373
Plasmid#138243PurposeExpresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R366
Plasmid#138236PurposeExpresses the split mCherry reporter interrupted by the LLP-Pol intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R367
Plasmid#138237PurposeExpresses the split mCherry reporter interrupted by the LP-Hel intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R356
Plasmid#138226PurposeExpresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
NDV-HN-SpyTag
Plasmid#160000PurposeExpresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cellsDepositorInsertNDV-HN-SpyTag
TagsHis6-tagExpressionMammalianPromoterChicken beta-actin promoterAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 UBXN11
Plasmid#169017PurposeExpresses GST-UBXN11 (human) in bacteriaDepositorAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
Plasmid#204288Purposeyeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORFDepositorInsertpHIS3p:yomRuby3-Tub1+3'UTR::LEU2
TagsmRuby3ExpressionYeastPromoterpHis3Available SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MitoRlucN-T2A-RlucCER-IRES-mCherry
Plasmid#249563PurposeFor detection of ER-mitochondrial contactDepositorInsertMitoRlucN-T2A-RlucCER
UseLuciferase and Synthetic BiologyExpressionMammalianPromoterChicken beta-actin promoter with CMV enhancerAvailable SinceDec. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM111
Plasmid#248760PurposeTetR part vector for Golden Gate AssemblyDepositorInsertPBfP2E5-RBS1PLP-TetR-rrnBT1
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM113
Plasmid#248761PurposeLacI part vector for Golden Gate AssemblyDepositorInsertPBfP2E5-RBS1PLP-LacI-rrnBT1
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM118
Plasmid#248762PurposePhlF part vector for Golden Gate AssemblyDepositorInsertPBfP2E5-RBS1PLP-PhlF-rrnBT1-T7Te
ExpressionBacterialAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFP.R362
Plasmid#138232PurposeExpresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R376
Plasmid#138246PurposeExpresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only