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Plasmid#209242PurposeMammalian expression of ATP8B2 mutant E171QDepositorInsertATP8B2 (ATP8B2 Human)
UseTagsHAExpressionMammalianMutationE171QPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP11B-HA
Plasmid#209236PurposeMammalian expression of ATP11BDepositorInsertATP11B (ATP11B Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP8A1-HA
Plasmid#209225PurposeMammalian expression of ATP8A1DepositorInsertATP8A1 (ATP8A1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N3-hARL1(Q71L)
Plasmid#206407PurposeMammalian expression of hARL1(Q71L)DepositorInsertARL1 (ARL1 Human)
UseTagsEGFPExpressionMammalianMutationQ71LPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-hARL1(T31N)
Plasmid#206408PurposeMammalian expression of hARL1(T31N)DepositorInsertARL1 (ARL1 Human)
UseTagsEGFPExpressionMammalianMutationT31NPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-27877
Plasmid#202110PurposeLevel 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert27877 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_CE-OriT
Plasmid#202150PurposeLevel 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.DepositorInsertOrigin of transfer
UseSynthetic BiologyTagsExpressionMutationOriT sequence mutated to remove sapI sites domest…PromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-Fld
Plasmid#202125PurposeLevel 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertFld promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-54730
Plasmid#202109PurposeLevel 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert54730 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-51183
Plasmid#202108PurposeLevel 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert51183 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-GEF
Plasmid#202107PurposeLevel 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertGEF promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-47740
Plasmid#202106PurposeLevel 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert47740 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI site for…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-BCA
Plasmid#202105PurposeLevel 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertBCA promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-epyC
Plasmid#202104PurposeLevel 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertepyC promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-flag-Lag16-fibcon-iCAM1-tether
Plasmid#205213PurposeThis vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-MOORmut
Plasmid#198213PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tagDepositorInsertsfGFP_MOORmut
UseTags6x His Tag and mutated (non-permissible) MOORmut …ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-mini
Plasmid#196338PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only