Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,821 results for: Por

Showing: 10001 - 10020 of 17821 results

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pLV-puro-EF1A-3XFLAG-hASB8

Plasmid

#242464

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-Tag100-hASB8

Plasmid

#242460

Purpose

Expresses human ASB8 protein with an N-terminal Tag100 along with a puromycin selection marker.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

Tag100

Expression

Mammalian

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-ITGB1(no stop)

Plasmid

#215453

Purpose

Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway Cloning

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

Sept. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-PTPN11(D425A,C459S)-Clover

Plasmid

#215526

Purpose

Expression vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Tags

Clover

Expression

Mammalian

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

EF1a

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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proUBI10::VIH2-FL::FLAG-H505A-H741A-H975A

Plasmid

#238337

Purpose

proUBI10::VIH2-FL::FLAG-H505A-H741A-H975A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-H505A-H741A-H975A

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A

Plasmid

#238333

Purpose

proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-R372A-H373A-R376A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A

Plasmid

#238338

Purpose

proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-P378V-G500V-G501A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A

Plasmid

#238339

Purpose

proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-R722S-D724A-T726A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A

Plasmid

#238340

Purpose

proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-K383A-K447A-K598A-R722A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A

Plasmid

#238334

Purpose

proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-R372A-H373A-R376A-E882A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A

Plasmid

#238341

Purpose

proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-K379A-K381A-K498A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235301

Purpose

ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2-bGH

Plasmid

#235300

Purpose

ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235302

Purpose

ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(YYFF)

Plasmid

#215451

Purpose

Gateway entry vector with integrin beta1 NPxY mutant (YYFF)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway Cloning

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MKC84_HBB(HBA1reg-HBA1)

Plasmid

#232410

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC83_HBB(HBBreg-HBA1)

Plasmid

#232409

Purpose

AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-P577S-V5/HIS

Plasmid

#234760

Purpose

Expression of the P577S mutant variant of human PDGFRA, which might be associated with Melanoma

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA-P577S receptor tyrosine kinase, full length (PDGFRA Human)

Tags

V5/His

Expression

Mammalian

Mutation

P577S substitution

Promoter

CMV

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-R487L-V5/HIS

Plasmid

#234761

Purpose

Expression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal Tumor

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)

Tags

V5/His

Expression

Mammalian

Mutation

R487L substitution

Promoter

CMV

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-PDGFRA-G853D-V5/HIS

Plasmid

#234757

Purpose

Expression of the G853D mutant variant of human PDGFRA, which might be associated with Melanoma

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA-G853D receptor tyrosine kinase, full length (PDGFRA Human)

Tags

V5/His

Expression

Mammalian

Mutation

G853D substitution

Promoter

CMV

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 10001 - 10020 of 17821 results

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