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pYPQ146

Plasmid

#69298

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRAM18dSFA[MCS]

Plasmid

#84685

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Article

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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23 pCOLI_A_N-SUMO_C_STREP

Plasmid

#160259

Purpose

N-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII, 6xHis, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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p(U6a-BsaI-gRNA)

Plasmid

#65955

Purpose

Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Type

Empty backbone

Expression

Insect

Promoter

U6

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pdestDD04Neo

Plasmid

#26386

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d24EGFP

Plasmid

#41944

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA-BfuAI-2k

Plasmid

#112915

Purpose

Empty sgRNA expression plasmid

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCoofy9

Plasmid

#122004

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

StrepII

Expression

Bacterial

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T

Plasmid

#64957

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRAM18dSGK[MCS]

Plasmid

#84684

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Article

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBLO 9.3_bCas9-CP230

Plasmid

#121497

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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RV-L3-HyTK-2L

Plasmid

#11684

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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pIIIA/MS2-2

Plasmid

#220632

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-N-Strep2

Plasmid

#119758

Purpose

Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.

Depositor

Andrew Jackson, Martin Reijns

Type

Empty backbone

Tags

GST and Strep-tag II fusion tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pACRISPR-CM

Plasmid

#203588

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Article

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Expression

Bacterial

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGGA000

Plasmid

#48856

Purpose

Empty entry vector for creating GreenGate promoter modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCoofy51

Plasmid

#122005

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

TwinStrep

Expression

Insect

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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p361_DG

Plasmid

#234683

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pNGFP-BC

Plasmid

#161956

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Tags

GFPuv His8

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCGFP-BC

Plasmid

#161959

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Tags

GFPuv His8

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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