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pT7-V5-SBP-C1-Hs4EBP1-Y54AL59A_Z

Plasmid

#148145

Purpose

Mammalian Expression of Hs4EBP1-Y54AL59A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EBP1-Y54AL59A (EIF4EBP1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EBP1-YLLVtoAAAA_Z

Plasmid

#148146

Purpose

Mammalian Expression of Hs4EBP1-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EBP1-YLLVtoAAAA (EIF4EBP1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SFRS2IP

Plasmid

#156012

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS2IP (SCAF11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SFRS17A

Plasmid

#156010

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS17A (SFRS17A Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pVPSBA01-SARS-CoV2-nsp1

Plasmid

#192171

Purpose

express SARS2-NSP1

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pVPSBA01-SARS-CoV2-nsp1 mutant4

Plasmid

#192174

Purpose

express SARS2-NSP1-mut4

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1-mut4 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pVPSBA01-SARS-CoV2-nsp1-truncated

Plasmid

#192172

Purpose

express SARS2-NSP1-trunc

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1-trunc (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Mp-iPGM

Plasmid

#180243

Purpose

Bacterial expression plasmid for production of recombinant Mycoplasma pneumoniae iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Mycoplasma pneumoniae iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R3tdTomatoi2

Plasmid

#185969

Purpose

Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick

Use

Synthetic Biology

Promoter

PR

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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