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JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

Plasmid

#104570

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x3)

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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hASXL(1-115)-luciferase

Plasmid

#106427

Purpose

Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

addition sex combs like 1 ASXL1 (ASXL1 Human)

Tags

luciferase

Expression

Mammalian

Mutation

amino acids 1-115 fused to luciferase

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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UHMK1 gRNA (BRDN0001147576)

Plasmid

#77184

Purpose

3rd generation lentiviral gRNA plasmid targeting human UHMK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

UHMK1 (UHMK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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PHKA2 gRNA (BRDN0001146244)

Plasmid

#77842

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKA2 (PHKA2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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NADK gRNA (BRDN0001146052)

Plasmid

#78073

Purpose

3rd generation lentiviral gRNA plasmid targeting human NADK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NADK (NADK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PCK2 gRNA (BRDN0001145460)

Plasmid

#78080

Purpose

3rd generation lentiviral gRNA plasmid targeting human PCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PCK2 (PCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PCK2 gRNA (BRDN0001145524)

Plasmid

#78081

Purpose

3rd generation lentiviral gRNA plasmid targeting human PCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PCK2 (PCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PCK2 gRNA (BRDN0001487084)

Plasmid

#78082

Purpose

3rd generation lentiviral gRNA plasmid targeting human PCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PCK2 (PCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK3 gRNA (BRDN0001145624)

Plasmid

#78039

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK3 (TSSK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK3 gRNA (BRDN0001487064)

Plasmid

#78040

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK3 (TSSK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK3 gRNA (BRDN0001162436)

Plasmid

#78041

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK3 (TSSK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TSSK3 gRNA (BRDN0001162469)

Plasmid

#78042

Purpose

3rd generation lentiviral gRNA plasmid targeting human TSSK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TSSK3 (TSSK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MST1R gRNA (BRDN0001487060)

Plasmid

#78050

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MST1R gRNA (BRDN0001145791)

Plasmid

#78051

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MST1R gRNA (BRDN0001145169)

Plasmid

#78052

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTBK2 gRNA (BRDN0001146661)

Plasmid

#77970

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTBK2 (TTBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTBK2 gRNA (BRDN0001148497)

Plasmid

#77971

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTBK2 (TTBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001149537)

Plasmid

#77927

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001487065)

Plasmid

#77928

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ROR2 gRNA (BRDN0001148055)

Plasmid

#77939

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROR2 (ROR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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