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pET-SUMO-SHP2-N-SH2(T42A)-Avi

Plasmid

#245183

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

T42A

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(WT)-Avi

Plasmid

#245182

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

wild-type

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-C-SH2(R138Q)-Avi

Plasmid

#245189

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 C-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

R138Q

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-3AF

Plasmid

#245785

Purpose

Expression of mutated bovine arrestin-3 (I385A, V386A, F387A) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

I385A, V386A, F387A

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS1

Plasmid

#239460

Purpose

For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci. Adv., 11, eadw9153 (2025)

Type

Empty backbone

Tags

VENUS:3xHA:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(E76K)-Avi

Plasmid

#245187

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

E76K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-C-SH2(WT)-Avi

Plasmid

#245188

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 C-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

wild-type

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Aga2p-TEVcs-LacAnc100-myc_pCTCON2

Plasmid

#245298

Purpose

expresses LaccID on the yeast surface

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

Aga2p-LacAnc100

Expression

Yeast

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_SS-HA-V5-LacAnc100-CD4 TM_pDisplay

Plasmid

#245299

Purpose

expresses LaccID on the mammalian cell surface

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LacAnc100-CD4TM

Expression

Yeast

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-C-SH2(E139D)-Avi

Plasmid

#245190

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 C-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

E139D

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS

Plasmid

#244842

Purpose

Bacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b PhiC31 attP41

Plasmid

#242881

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 4a PhiC31 attP41

Plasmid

#242882

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 3b PhiC31 attP41

Plasmid

#242883

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b Bxb1 attB38

Plasmid

#242879

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a Bxb1 attB38

Plasmid

#242878

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a PhiC31 attP41

Plasmid

#242880

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Nsp3(1-111)

Plasmid

#242947

Purpose

GFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)

Depositor

Benjamin Schuster

Article

Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

Insert

GFP-Nsp3(1-111) (ORF1ab SARS-CoV-2)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-APOX-KDEL

Plasmid

#242339

Purpose

Expresses human APOX in ER/Golgi

Depositor

Jeffrey Martell

Article

Fang et al ChemRxiv. 2025; doi:10.26434/chemrxiv-20

Insert

APOX-FLAG-KDEL (APEX2 Human)

Tags

FLAG and KDEL

Expression

Mammalian

Mutation

F41V, S69I, Y235F, H239Y

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-APOX

Plasmid

#242344

Purpose

Expresses human APOX in cytosol

Depositor

Jeffrey Martell

Article

Fang et al ChemRxiv. 2025; doi:10.26434/chemrxiv-20

Insert

FLAG-APOX (APEX2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F41V, S69I, Y235F, H239Y

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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