We narrowed to 34,123 results for: CMV

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pTE_19 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-NLS-HbaUTR

Plasmid

#252015

Purpose

In vitro transcription of Cas9 only for mRNA generation

Depositor

Neville Sanjana

Publication

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1

Plasmid

#250914

Purpose

Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines

Depositor

Philipp Niethammer

Publication

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-3xNLS-P2A-EGFP-LMNB1 (lmnb1, Arfgap1 Rat, Zebrafish)

Tags

EGFP on LMNB1 and mKate2 on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 196–234 only

Promoter

CMV

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Plasmid

#233424

Purpose

IVT template for LIDAR reporter mRNA; EGFP as marker, Cre recombinase as output

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Publication

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

Plasmid

#199346

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP

Plasmid

#179636

Purpose

TEVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Publication

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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R713-M88-303: CMV51p> Halotag-Hs.HRAS

Plasmid

#159681

Purpose

Mammalian protein expression of HRAS-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.HRAS (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBLO44.1_dProtease

Plasmid

#121631

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

TEV protease

Expression

Mammalian

Promoter

U6 and chicken beta-actin promoter

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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mito cpVenus control

Plasmid

#186792

Purpose

Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Publication

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

mito cpVenus control

Use

Lentiviral

Expression

Mammalian

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAL204

Plasmid

#22282

Purpose

Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV10-VPS13C-W374K I378D L382K-GFP-3xFLAG

Plasmid

#248324

Purpose

Mammalian expression of VPS13C mutant for in vitro studies

Depositor

Karin Reinisch

Publication

Li et al Mol Cell. 2026 Jul 7:S1097-2765(26)00417

Insert

VPS13C (VPS13C Human)

Tags

GFP-3xFLAG

Expression

Mammalian

Mutation

W374K, I378D, L382K

Promoter

CMV

Available since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV10-VPS13C-W374K W393D W395K-GFP-3xFLAG

Plasmid

#248325

Purpose

Mammalian expression of VPS13C mutant for in vitro studies

Depositor

Karin Reinisch

Publication

Li et al Mol Cell. 2026 Jul 7:S1097-2765(26)00417

Insert

VPS13C (VPS13C Human)

Tags

GFP-3xFLAG

Expression

Mammalian

Mutation

W374K, W393D, W395K

Promoter

CMV

Available since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-IRES2-CRY2-STIM1(238-448)

Plasmid

#248295

Purpose

An expression construct encoding a CRY2-fused STIM1 fragment (a.a.238-448) linked to EGFP through an IRES2 element.

Depositor

Sangkyu Lee

Publication

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

EGFP and CRY2-fused STIM1 fragment (a.a.238-448) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-237,449-685

Promoter

CMV

Available since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

Plasmid

#223134

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

Plasmid

#223135

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9(KWRQLC)-P2A-EGFP (BKS1026)

Plasmid

#223126

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(KWRQLC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/KWRQLC)=D1135K, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9(LWRYEK)-P2A-EGFP (BKS1022)

Plasmid

#223128

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(LWRYEK)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/LWRYEK)=D1135L, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9(MKRCKS)-P2A-EGFP (RAS3445)

Plasmid

#223130

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MKRCKS)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MKRCKS)=D1135M, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(ARGIMR)-P2A-EGFP (BKS1036)

Plasmid

#223132

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(ARGIMR)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/ARGIMR)=D1135A, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(MRKCRS)-P2A-EGFP (AHK545)

Plasmid

#223133

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKCRS)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKCRS)=D1135M, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge