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pGL2_162

Plasmid

#199094

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pUB110 ORI plus NGS barcode 2, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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AM77_pU6.Sa-gRNA.CLYBL

Plasmid

#199237

Purpose

Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Publication

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. aureus gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-PFN2_variant2

Plasmid

#192300

Purpose

Gateway entry vector for an inducible 3xFLAG-PFN2_variant2

Depositor

Kevin Janes

Publication

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

PFN2_variant2 (PFN2 Human)

Use

Gateway Cloning

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-TPI-wildtype-TREAT

Plasmid

#196923

Purpose

The coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORF

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-miR21-SunTag-TREAT

Plasmid

#196932

Purpose

SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CoA-Snifit(G41)

Plasmid

#197945

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Publication

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-VD2

Plasmid

#193419

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Publication

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-VD4

Plasmid

#193421

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Publication

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-VD3

Plasmid

#193420

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Publication

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-VD1

Plasmid

#193418

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Publication

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ

Plasmid

#192387

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP759_L2pV1_OR-OCS_0I

Plasmid

#192418

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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TOB1

Plasmid

#156089

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TOB1 (TOB1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DHX40

Plasmid

#155540

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX40 (DHX40 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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RBM38

Plasmid

#155899

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM38 (RBM38 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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RBBP6

Plasmid

#155879

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBBP6 (RBBP6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PLRG1

Plasmid

#155827

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PLRG1 (PLRG1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIRSTD)-sfGFP

Plasmid

#189674

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Publication

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-LjCYP722C(pYL795)

Plasmid

#178292

Purpose

Expresses LjCYP722C from L. japonicus in Saccharomyces cerevisiae. Centromeric TRP, PGPD-LjCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

LjCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A hu_ncOGT WT (open N-term)

Plasmid

#155197

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase with open N-terminus

Depositor

Suzanne Walker

Publication

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Mutation

WT OGT with no start codon, in-frame with gateway…

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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