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PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B[T22N]

Plasmid

#24909

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8B

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 SSA1

Plasmid

#19516

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAK

Plasmid

#19517

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

dnaK molecular chaperone DnaK

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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PA1024_pET21a(+)C-tag

Plasmid

#14579

Depositor

SeWon Suh

Article

Ha et al J Biol Chem. 2006 Jul 7. 281(27):18660-7

Insert

2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pET28g

Plasmid

#249630

Purpose

Clone genes for protein expression in Escherichia coli using Golden Gate cloning and the pET28a backbone

Depositor

Isabel Abreu

Article

Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi

Type

Empty backbone

Expression

Bacterial

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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puDNA5_SSF_MOR_2Ala_APEX2

Plasmid

#250760

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5_TO_FRT_myc_GLUT4_2Ala_APEX2

Plasmid

#250767

Purpose

Mammalian cell expression of GLUT4 in which L500 and L503 are mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(2Ala)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Mutation

L500 and L503 mutated to alanines

Promoter

CMV

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250765

Purpose

Mammalian cell expression of an shRNA against VPS35 and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

VPS35 shRNA

Tags

SSF-SNAP(for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250764

Purpose

Mammalian cell expression of a scrambled shRNA and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

Scrambled VPS35 shRNA

Tags

SSF-SNAP (for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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