We narrowed to 1,254 results for: sumo

1. PAS_RvSAHS4

   Plasmid

   #215646

   Purpose

   Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS4
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-UBC9-K14R-A129K

   Plasmid

   #228948

   Purpose

   express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   changed Lysine 14 to Arginine, changed Alanine 12…

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-RANGAP1-CTD

   Plasmid

   #228947

   Purpose

   express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized

   Depositor

   Marcin Suskiewicz

   Insert

   RANGAP1-CTD (RANGAP1 Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGSSHHHHHHS…

   Expression

   Bacterial

   Mutation

   contains aa 418-587 only

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPIH6

   Plasmid

   #190676

   Purpose

   E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

   Depositor

   John Vederas

   Article

   Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

   Inserts

   Small ubiquitin-like modifier
   Mxe GyrA intein with C-terminal chitin binding domain and His tag
   

   Use

   Tags

   Chitin binding domain, His tag, and N-terminal Hi…

   Expression

   Bacterial

   Mutation

   Added C-terminal his tag and None

   Promoter

   T7

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAIP4

   Plasmid

   #233686

   Purpose

   Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

   Depositor

   Christopher Bahl

   Insert

   Smt3 (SMT3 )
   

   Use

   Tags

   HisTag

   Expression

   Mutation

   Promoter

   T7

   Available Since

   April 7, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. PML-3/pSG5

   Plasmid

   #50939

   Purpose

   expression of PML with Sumo sites destroyed

   Depositor

   Pier Pandolfi

   Article

   Zhong et al Blood. 2000 May 1;95(9):2748-52.

   Insert

   PML-3 (PML Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K65R, K160R, K490R (Sumo modification sites)

   Promoter

   SV40

   Available Since

   Jan. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pSC-hTDG-K330A-PreE2

   Plasmid

   #52273

   Purpose

   Bacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   hTDG (TDG Human)
   

   Use

   Tags

   Ubc9-GST

   Expression

   Bacterial

   Mutation

   K330A, SUMO acceptor site mutation

   Promoter

   T7

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC-hTDG-K330A-IntE2

   Plasmid

   #52271

   Purpose

   Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein, SUMO-E2 and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   hTDG (TDG Human)
   

   Use

   Tags

   GyrA-intein-Ubc9-GST

   Expression

   Bacterial

   Mutation

   K330A, SUMO acceptor site mutation

   Promoter

   T7

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC-hXRCC1-K176R-IntE2

    Plasmid

    #52277

    Purpose

    Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GyrA-intein-Ubc9-GST

    Expression

    Bacterial

    Mutation

    K176R, SUMO acceptor site mutant

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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