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Plasmid#176305PurposeExpresses PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
UseTagsHA tagExpressionInsectMutationE113K, N265S- please depositor commentsPromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/NLS-mChe
Plasmid#183162PurposeConstruct for transient expression of cell nucleus-targeted mCherry in plants.DepositorInsertNLS-mCherry
UseTags16 aa nuclear localization signal and 16 aa nucl…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Mito-mChe
Plasmid#183167PurposeConstruct for transient expression of mitochondrion-targeted mCherry in plants.DepositorInsertMito-mCherry
UseTagsOsCOX11, Oryza sativa cytochrome c oxidase assemb…ExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vimentin-C-18
Plasmid#55157PurposeLocalization: Intermediate Filaments, Excitation: 587, Emission: 610DepositorInsertVimentin (VIM Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
UseTagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a-EGFP
Plasmid#205303PurposeExpression of WHaloCaMP1a-EGFP fusion in E. coliDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHDE-35S-Cas9-mCherry
Plasmid#78931PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plantsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19/ER-mChe
Plasmid#183163PurposeConstruct for transient expression of endoplasmic reticulum-targeted mCherry in plants.DepositorInsertER-mCherry
UseTags25 aa ER signal of Glycine soja kunitz-type tryps…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-G97 IRES EGFP
Plasmid#130711PurposePlasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-G97 IRES EGFP
UseTagsLyn11 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-F98 IRES EGFP
Plasmid#130710PurposePlasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-F98 IRES EGFP
UseTagsLyn11 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
P445-FBP_17
Plasmid#139708PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlledDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P447-FBP_19
Plasmid#139710PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsiveDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-p24
Plasmid#130718PurposePlasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid proteinDepositorInsertSNAP-Tag-p24
UseTagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ827
Plasmid#155321PurposeConstitutive expression of E-PIF6(1-100) in plantsDepositorInsertPCaMV35S-E-PIF6(1-100)-NLS-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF051
Plasmid#155328PurposeConstitutive expression of SRDX-EL222 in plantsDepositorInsertPCaMV35S-SRDX-NLS-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES EGFP
Plasmid#130706PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES EGFP
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrie…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBI101.1/ProGOXL1:GUS
Plasmid#175557PurposeBinary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genesDepositorInsertProGOXL1
UseTagsGUSExpressionPlantMutationPromoterGOXL1Available sinceJan. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-EF1-IRES-NEO deleted ITR
Plasmid#67906PurposePiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeatDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Vimentin-N-18
Plasmid#57690PurposeLocalization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertNM_003380.3 (VIM Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICSL11023
Plasmid#117542PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S_OMEGA(pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-CT
Plasmid#18069DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
UseTagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0113
Plasmid#117552PurposeLevel 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES mYFP
Plasmid#130713PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-G97-NLS IRES mYFP
UseTagsNLS and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only