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FLAG-Stx17

Plasmid

#45911

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pSBbi-Neo

Plasmid

#60525

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-pLtetO-gfp

Plasmid

#89476

Purpose

Broad-host plasmid expressing gfp under pLtetO promoter

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pLtetO-GFPmut3b

Use

Tags

Expression

Mutation

Promoter

pLtetO

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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HS-Ras-mKate2

Plasmid

#105951

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Ras

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSB_syb25

Plasmid

#197992

Purpose

Expressed sybody 25 as a C-terminal myc-His-tagged construct

Depositor

Simon Newstead

Article

Parker et al Nat Struct Mol Biol. 2023 Jul 23. doi: 1

Insert

Sybody 25

Use

Tags

Myc-His

Expression

Bacterial

Mutation

Promoter

Arabinose

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-RN

Plasmid

#60519

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG tubulin GFP

Plasmid

#66105

Purpose

expression of chicken tubulin GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

tubulin

Use

Chicken expression

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
Skylan-S-N1

Plasmid

#129737

Purpose

A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

Depositor

Pingyong Xu

Article

Zhang et al ACS Nano. 2015 Mar 24;9(3):2659-67. doi:

Type

Empty backbone

Use

Tags

Skylan-S

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and mouse U6

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSBbi-BN

Plasmid

#60518

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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PX853

Plasmid

#62885

Purpose

N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-573)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Promoter

CBh

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-rxRFP

Plasmid

#67830

Purpose

Redox-Sensitive Red Fluorescent Protein

Depositor

Huiwang Ai

Article

Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

Insert

Redox-Sensitive Red Fluorescent Protein

Use

Tags

His Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBN41

Plasmid

#86716

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-2::GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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HS-mKate2

Plasmid

#105945

Purpose

heat shock inducible transgenesis, cytosolic mKate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

mKate2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAG506-mito-frFAST

Plasmid

#160057

Purpose

Expresses mito-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

Mito-frFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pU1utr-EGFP

Plasmid

#140268

Purpose

U1A-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

U1A binding site

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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plet7d_stbC-EGFP

Plasmid

#140266

Purpose

LIN28-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Stabilized hairpin of miR-let7d

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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HPGD

Plasmid

#39154

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

HPGD (HPGD Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pNCS-Flag

Plasmid

#17364

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2003 Feb . 77(3):1820-9.

Insert

MMLV proviral DNA

Use

Tags

Flag

Expression

Mutation

Promoter

Available since

March 1, 2010

Availability

Academic Institutions and Nonprofits only