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HA-Rab8a-WT

Plasmid

#101048

Purpose

Expresses HA-tagged human Rab8-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pB.GFP-KIF1A tail 395-1698

Plasmid

#134618

Purpose

Mammalian expression of mouse KIF1A 395-1698

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1A (Kif1a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 394 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF1C tail 400-1103

Plasmid

#134621

Purpose

Mammalian expression of human KIF1C 400-1103

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1C (KIF1C Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-n1-AICD

Plasmid

#69925

Purpose

mammalian expression of human AICD fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

47 aa long

Promoter

CMV

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

Plasmid

#134613

Purpose

Mammalian expression of rat KIF5C 378-955

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5C (Kif5c Rat)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmReNL

Plasmid

#182970

Purpose

Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmReNL

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE puro Tusc5

Plasmid

#87952

Purpose

retroviral plasmid containing mouse Tusc5

Depositor

David James

Article

Fazakerley et al J Biol Chem. 2015 Sep 25;290(39):23528-4

Insert

Tumor Suppressor Candidate 5 (Tusc5 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-PSTCD-cGAS

Plasmid

#102605

Purpose

Mammalian expression of cGAS fused to PSTCD tag

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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VAMP7 HA-delta pMEP4

Plasmid

#62958

Purpose

full length human VAMP7 with C-terminal HA tag cloned into delta pMEP4

Depositor

Paul Luzio

Article

Pryor et al Cell. 2008 Sep 5;134(5):817-27. doi: 10.

Insert

VAMP7 (VAMP7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pGAPTrap TALEN 2

Plasmid

#83369

Purpose

Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-Syn1-DIO-VGLUT2:miniSOG

Plasmid

#214200

Purpose

For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

Depositor

Thomas Hnasko

Article

Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

Insert

Slc17a6 tagged with miniSOG and hemagglutinin

Use

AAV and Cre/Lox

Tags

miniSOG (tagged with HA) is fused to the first in…

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-TALEN1

Plasmid

#83368

Purpose

Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN1

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
PB-Ef1a-RPS27A-UTR-PP7-WPRE

Plasmid

#198339

Purpose

PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

RPS27A-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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K15-EGFP

Plasmid

#44266

Depositor

George Cotsarelis

Article

Morris et al Nat Biotechnol. 2004 Apr;22(4):411-7. Ep

Insert

K15 promoter (-4.8) (Krt15 Mouse)

Use

Mouse Targeting; Fluorescence microscopy

Tags

EGFP

Expression

Mammalian

Mutation

Contains the murine K15 promoter (-4.8) fragment

Promoter

murine K15 promoter (-4.8) fragment

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
GFP-FKBP-TPD54

Plasmid

#172454

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Use

Tags

GFP-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
PB-Ef1a-SunTag-RPS7-PP7-WPRE

Plasmid

#198338

Purpose

PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

GCN4x24-RPS7-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops and SunTag

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-Myc

Plasmid

#177908

Purpose

human CD81-TEV cleavage site-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81 (CD81 Synthetic, Human)

Use

Tags

FKBP, Myc tag, and TEV protease cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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