We narrowed to 395 results for: ICL

...your article doesn’t turn into a textbook. Get the journal’s submission rules for review articles Whether...from the article to be able to keep editing it effectively. You will also begin to hate the article. This...Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you ...basically, a scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser... good” by no definitive criteria. I read these articles to get a sense of the themes in the field and ...for seminal papers on these themes, and also for articles from the active/well-known researchers in the ...feedback on the scope and the content. Submit your article! Overall, writing a review can be overwhelming ...

...of citing articles. From the article list you can click on the PubMed link to find the article abstract...from these citing articles. What's this? Simply put, if the authors of a "Citing Article" have deposited... a new feature on our plasmid pages - links to articles citing this plasmid. Now you can learn how a plasmid...publications, you'll find a link to the list of citing articles under the "Resource Information" heading in the...heading, you'll be directed to a page listing the articles that cite this plasmid. You can use the dropdown...deposited plasmids associated with this "Citing Article" - such as cloning their gene of interest into a previously...from Addgene may get your paper listed in the "Articles Citing This Plasmid" list increasing your work's...

...Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles F. Infecting...for producing viral particles. pMD2.G Envelope plasmid for producing viral particles. Note: pLKO.1 can ...selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence of ...transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line...sequence. Back to Top E. Producing Lentiviral Particles Before this step, you must contact your institution...Addgene recommends that you generate lentiviral particles and infect the target cells. Addition of puromycin...Tools page. E.2 Protocol for Producing Lentiviral Particles This protocol is for transfection in a 6 cm plate...

...B): LiCl selectively precipitates RNA versus DNA or proteins. Add the correct amount of 7.5 M LiCl solution...solution to bring the concentration of LiCl in the extracted aqueous layer to 2.5 M. Incubate at -20°C ...

...for the presence of particles still in solution. Continue to stir until all particles have dissolved. This...stir on a magnetic stir plate. Stir until all particles have dissolved. Note: The powder should dissolve...

...containing) particles. On average, iodixanol-purified AAV preparations contain ~20% empty particles. 60% iodixanol...

...proteins, cell fragments, salt and other extra particles from solutions. Supernatant contains the plasmid...may appear, consisting of precipitated protein particles Bottom phase - Organic phase (protein) Pipet the...

...too many cells relative to the number of virus particles reduces the transduction efficiency, resulting... so many that they vastly outnumber the virus particles. Incubate the cells with the virus for 48–72 h...

...Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology,... to determine the number of genome-containing particles of an AAV prep using SYBR green technology. This...

...generally represents the number of infectious viral particles. Users may need to run lower or higher dilutions... zero (A01). In this protocol, the lentiviral particles are serially diluted and used to transduce HEK293T...

...lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles See Addgene's...) are packaged as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on...on the envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that ...infective potential. The following types of viral particles have been pseudotyped with the SARS-CoV-2 Spike...; several modifications may enhance infectious particle production, including codon optimization, deletion...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped...

...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging. Neuron. 2009 Feb 12...temporal cellular processes. Note that many of the articles linked below include variants with different binding...Request for many more. Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded Calcium...Return to top Small Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor for bacterial...Return to top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors (GACh) based...plasma membrane Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff...

...then are transported to the Golgi apparatus in vesicles to be packaged and shipped. On the other hand,... membrane-bound structures and brings membrane vesicles and protein cargo from the cell surface via endosomes... Maria Harrison 14435 mRFP-Clc Clathrin-coated vesicles Clathrin light chain mRFP Ari Helenius 54040 mEmerald-Clathrin... mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin light chain mEmerald* Michael Davidson 79997 pmiRFP703... pmiRFP703-Clathrin Clathrin-coated vesicles Clathrin light chain miRFP703 Vladislav Verkhusha 21741 Clathrin-LCa-EYFP... Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin light chain EYFP Xiaowei Zhuang 164462 Clathrin-mGreenLantern...-mGreenLantern Clathrin-coated vesicles Clathrin light chain mGreenLantern Gregory Petsko 184667 pClathrin-miRFP670nano3...

...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale...produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using...of empty to full (i.e., genome containing) AAV particles within representative vector preparations was ...microscopy after negative staining. Empty vector particles can be identified after negative staining and ...and appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows...

...(DOCX, 184 KB) Research Articles Many publishers allow free access to articles related to SARS-CoV-2 research..., we have linked to collections of open-access articles, protocols, and other resource collections related...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped...window) Springer Nature SARS-CoV-2 and COVID-19 Articles (Link opens in a new window) Wiley Online Library...

...Plasmids can be found associated with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila...N-terminal epitope tags. The plasmids in the following articles provide PCR templates for amplification of the...can be found associated with the following two articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic...Plasmids can be found associated with the following article: Paix, et al. Genetics 2014 Allen Institute for...Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, ...

...Lentiviruses are distributed as purified viral particles in solution that are suitable for in vivo use....Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library...Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library...Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library...

...select the article link to find more information. Delivery Method Species Description Article PI Lentivirus...the article link to find more information. Convert From Convert To Delivery Method Species Article PI ...

...Lentiviral Tet-On vector for inducible expression. See article ( Meerbrey et al., 2011 ) for additional tookit...transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional selection...inducible expression of transgene and/or shRNA. See article ( Szulc et al., 2006 ) for Tet-Off and additional...vector, expresses tTA from the CAG promoter. See article ( Chan et al., 2017 ) for other viral tTA plasmids...

...publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available...publication link to view all other plasmids from that article. Click on the PI link to view all available plasmids...