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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8 results for: ICL

Showing: 1 - 8 of 8 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ...packaging genes gag , pol , and env to create viral particles. Wild-type lentiviruses additionally require the...lentivirus production — that is, creation of viral particles that could infect cells and further replicate ...concentrate. You can use crude or concentrated viral particles to transduce the cells of interest, either with...types and enhances the stability of the viral particles. However, VSV-G is toxic under long-term, constitutive...cell genome but cannot produce functional viral particles without the genes encoded in the envelope and ...the resulting construct is packaged into a viral particle, it is not possible for viral vectors to replicate...Precursor structural protein of the lentiviral particle containing matrix, capsid, and nucleocapsid components...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... change, the supernatant containing the viral particles is removed and stored or centrifuged to concentrate...types and enhances the stability of the viral particles. However, VSV-G is toxic under long-term, constitutive...Gamma-retroviral vectors have been shown to be safe delivery vehicles and provide stable integration of the intended...cell genome but cannot produce functional viral particles without the genes encoded in the envelope and ...that resulting construct is packaged into a viral particle, it is not possible for viral vectors to replicate...Precursor structural protein of the retroviral particle containing matrix, capsid, and nucleocapsid components...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...not have a lipid bilayer surrounding the viral particle and are only composed of a protein capsid and ...then act as in vitro factories for the new viral particles in the laboratory. The cell line used for rAAV...have also been developed. The newly formed viral particles produced in HEK293T cells are then extracted and...target cells, but cannot produce any new viral particles after the initial infection. The risk of spontaneously.... Anterograde transport Movement of molecules, vesicles or organelles from the main cell body, or soma... . Retrograde transport Movement of molecules, vesicles or organelles from the synapse at the axons towards...

  4. Chemogenetics Guide

    Type
    Guide
    ...respond specifically to the anti-smoking drug varenicline. This PSAM carries mutations at L131G, Q139L,...and is termed PSAM4 (or PSAM 4 ). Additional varenicline analogs, termed ultrapotent PSEMs (or uPSEMs)...Effect Outcome (in neurons) Reference PSAM4 Gly Varenicline, uPSEM 792, uPSEM 817 Cl - influx Neuronal inhibition...inhibition Magnus et al., 2019 PSAM4 5HT3 Varenicline, uPSEM 792, uPSEM 817 Na + , K + influx Neuronal...

  5. Molecular Biology Reference

    Type
    Guide
    ...to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect...service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn more. Regardless...

  6. Plan Your Experiment

    Type
    Guide
    ...provide the necessary components to make lentiviral particles (for details about lentivirus, see our Lentivirus... AAV transfer vector and used to generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging...

  7. Adenovirus Guide

    Type
    Guide
    ...not have a lipid bilayer surrounding the viral particle and are only composed of a protein capsid and ...a gRNA expression cassette in one single viral particle. Vaccine Development rAdV vectors are highly immunogenic...

  8. CRISPR Guide

    Type
    Guide
    ...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in vivo ...
Showing: 1 - 8 of 8 results