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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11 results for: ICL

Showing: 1 - 11 of 11 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles F. Infecting...for producing viral particles. pMD2.G Envelope plasmid for producing viral particles. Note: pLKO.1 can ...selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence of ...transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line...sequence. Back to Top E. Producing Lentiviral Particles Before this step, you must contact your institution...Addgene recommends that you generate lentiviral particles and infect the target cells. Addition of puromycin...Tools page. E.2 Protocol for Producing Lentiviral Particles This protocol is for transfection in a 6 cm plate...

  2. Kit Free RNA Extraction

    Type
    Protocol
    ...B): LiCl selectively precipitates RNA versus DNA or proteins. Add the correct amount of 7.5 M LiCl solution...solution to bring the concentration of LiCl in the extracted aqueous layer to 2.5 M. Incubate at -20°C ...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...for the presence of particles still in solution. Continue to stir until all particles have dissolved. This...stir on a magnetic stir plate. Stir until all particles have dissolved. Note: The powder should dissolve...

  5. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...proteins, cell fragments, salt and other extra particles from solutions. Supernatant contains the plasmid...may appear, consisting of precipitated protein particles Bottom phase - Organic phase (protein) Pipet the...

  6. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...too many cells relative to the number of virus particles reduces the transduction efficiency, resulting... so many that they vastly outnumber the virus particles. Incubate the cells with the virus for 48–72 h...

  7. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology,... to determine the number of genome-containing particles of an AAV prep using SYBR green technology. This...

  8. Lentivirus ddPCR Titration

    Type
    Protocol
    ...generally represents the number of infectious viral particles. Users may need to run lower or higher dilutions... zero (A01). In this protocol, the lentiviral particles are serially diluted and used to transduce HEK293T...

  9. AAV ddPCR Titration

    Type
    Protocol
    ...generally represents the physical number of viral particles. Users may need to run lower or higher dilutions...

  10. AAV Production in HEK293 Cells

    Type
    Protocol
    ...filters made of materials other than PES. AAV particles stick to many other surfaces, but do not stick...

  11. CRISPR Library Amplification

    Type
    Protocol
    ... are not efficiently packaged into lentiviral particles. See below for options if the recombinant band...
Showing: 1 - 11 of 11 results