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pDEST-V5_Hu_p115-RhoGEF(WT)

Plasmid

#136335

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal V5

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer10b-EGFP-KRAS G12V

Plasmid

#164925

Purpose

Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

Depositor

Ji Luo

Article

Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

Inserts

Enhanced green fluorescent protein
KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Glycine 12 changed to valine (G->T 3156)

Promoter

Ubc Promoter and Ubc promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP_Hu_p115-RhoGEF(WT)

Plasmid

#136337

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MCU-Flag-TetOFF

Plasmid

#185657

Purpose

Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

pTight TRE

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pETM30-ARC105

Plasmid

#133426

Purpose

Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Use

Tags

Expression

Bacterial

Mutation

Contains amino acids 5-78 fused to C-terminal of …

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLXSN p200 CUX1

Plasmid

#90470

Purpose

Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

Depositor

Alain Nepveu

Article

Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

Insert

CUX1 (CUX1 Human)

Use

Retroviral

Tags

Ha and myc

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus long terminal repeat

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-MCU-mCerulean

Plasmid

#185809

Purpose

Human mitochondrial calcium uniporter with C-terminus mCerulean tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

Mitochondrial calcium uniporter (MCU Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-arc105

Plasmid

#133425

Purpose

Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Use

Tags

Expression

Bacterial

Mutation

Contains amino acids 1-95 or ARC105 fused to the …

Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti/TO/EYFP-P2A-IDH1(HA)

Plasmid

#122482

Purpose

A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

Depositor

Eric Huang

Article

Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

Insert

isocitrate dehydrogenase 1 (IDH1 Human)

Use

Lentiviral

Tags

HA and P2A

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 159-329

Plasmid

#140148

Purpose

GAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 159-329 (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 1 to 158 and 330 to 479

Promoter

EF1a

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-p110 CUX1

Plasmid

#100816

Purpose

Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

Depositor

Alain Nepveu

Article

Vadnais et al BMC Genomics. 2013 Apr 16;14:258. doi: 1

Insert

Hs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)

Use

Lentiviral

Tags

HA and myc

Expression

Mammalian

Mutation

contains amino acids 747-1505

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5WT-VA

Plasmid

#98688

Purpose

Lentiviral expression of human PRDX5-WT in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1WT

Plasmid

#83444

Purpose

Mammalian expression of SOD1WT

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133insTT

Plasmid

#83446

Purpose

Mammalian expression of SOD1E133insTT.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E133insTT (after GA)

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pPHAGE_DLD-H450A-C-TAP

Plasmid

#83478

Purpose

Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

H450A

Promoter

CMV

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pPHAGE_DLD-S456A-C-TAP

Plasmid

#83479

Purpose

Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

S456A

Promoter

CMV

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLXSN p110 CUX1

Plasmid

#90471

Purpose

Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively

Depositor

Alain Nepveu

Article

Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

Insert

CUX1 (amino acids 747-1505) (CUX1 Human)

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus long terminal repeat

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133Δ

Plasmid

#83445

Purpose

Mammalian expression of SOD1E133Δ.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E133Δ

Promoter

EF-1α

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133K-AcGFP1

Plasmid

#83442

Purpose

Expresses SOD1E133K in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

E133K

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse ZNG1-METAP1 fusion

Plasmid

#197398

Purpose

Expresses a SUMO-tagged fusion of ZNG1, residues 10-30 and mouse METAP1, residues 1-59

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1-METAP1 (Cbwd1, Metap1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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