We narrowed to 2,568 results for: PGK

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pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

Plasmid

#163177

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Nanog-pA-pgk-hph

Plasmid

#74915

Purpose

Mammalian expression of mNanog

Depositor

Jose Silva

Article

Theunissen et al Curr Biol. 2011 Jan 11;21(1):65-71. doi:

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-RAP2A-V12-neo

Plasmid

#156178

Purpose

Lentiviral vector for expression of RAP2A-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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LV UAS-DsRed-Express2-PGK-Puro

Plasmid

#190801

Purpose

LV construct encoding UAS-DsRed-Express2 reporter gene.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

UAS-T2A-DsRed-Express2

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Klf4-pA-pgk-hph

Plasmid

#74906

Purpose

Mammalian expression of mKlf4

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Klf4 (Klf4 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-EGFP

Plasmid

#66821

Purpose

dCas9 fused to BFP and EGFP

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-EGFP

Tags

dSpCas9-BFP-EGFP

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

Plasmid

#163178

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-coemiRFP670-PGK-Puro

Plasmid

#186168

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

emiRFP670

Use

Marmoset targeting

Mutation

human codon-optimized

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PGK-ZhcD-Cit-tevs-DHFR

Plasmid

#116051

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

ZhcD-Cit-tevs-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-N17-neo

Plasmid

#156179

Purpose

Lentiviral vector for expression of RAP2A-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pgk-DIO-rvCRY-WPRE

Plasmid

#182060

Purpose

Plasmids for production of AAV-based TFactivity reporter

Depositor

Kentaro Abe

Article

Abe et al iScience. 2022 Feb 14;25(3):103927. doi:

Insert

mCherry

Use

AAV

Promoter

hu Pgk1

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mScarletI. PGK-puro

Plasmid

#136266

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mScarletI

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-cNanog-pgk-hph

Plasmid

#74907

Purpose

Mammalian expression of cNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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PB-PGK-teD-Cit-hcvs-DHFR

Plasmid

#116050

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

teD-Cit-hcvs-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-PGK-tvD-Cit-tevs-DHFR

Plasmid

#116053

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

tvD-Cit-tevs-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Expression

Yeast

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-TdT-guide1-PGK-Puro

Plasmid

#102903

Purpose

EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

TdT-guide1-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag ADPGK

Plasmid

#20417

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ADPGK (ADPGK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLV-PGK-SNAP-MDMUT-SOX2

Plasmid

#115688

Purpose

Constitutive expression of a SOX2 protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

SNAP-MDMUT-SOX2

Use

Lentiviral

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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