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We narrowed to 1,100 results for: cas12a

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Showing: 1001 - 1020 of 1100 results

  1. pYPQ239-RR

    Plasmid
    #108862
    Purpose
    FnCpf1 Gateway entry plasmid with N623R and K687R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R and K687R, Cpf1 is rice codon optimized
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108862-plasmid-map-sequence-id-274177

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  2. pY112 (pcDNA3.1-huSaCpf1)

    Plasmid
    #92269
    Purpose
    Expression of humanized SaCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huSaCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92269-plasmid-map-sequence-id-181496

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  3. pY114 (pcDNA3.1-huPgCpf1)

    Plasmid
    #92273
    Purpose
    Expression of humanized PgCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huPgCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92273-plasmid-map-sequence-id-181493

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  4. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid
    #92298
    Purpose
    Expression of humanized CPbCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huCPbCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92298-plasmid-map-sequence-id-181648

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  5. pY118 (pcDNA3.1-huLb4Cpf1)

    Plasmid
    #92294
    Purpose
    Expression of humanized Lb4Cpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huLb4Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92294-plasmid-map-sequence-id-181497

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  9. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166729-plasmid-map-sequence-id-332207

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  10. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Availability
    Academic Institutions and Nonprofits only
    155386-plasmid-map-sequence-id-309003

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  11. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166730-plasmid-map-sequence-id-332216

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  13. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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  19. pYPQAT45

    Plasmid
    #223417
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223417-plasmid-map-sequence-id-453515

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  20. pYPQAT46

    Plasmid
    #223418
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223418-plasmid-map-sequence-id-454166

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Showing: 1001 - 1020 of 1100 results