We narrowed to 5,293 results for: cycs

1. pDT4

   Plasmid

   #199120

   Purpose

   scrambled promoter circular DNA template

   Depositor

   Jeff Gelles

   Article

   Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

   Insert

   synthetic transcription template
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE30-butelase1

   Plasmid

   #115430

   Purpose

   Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

   Depositor

   Joshua Mylne

   Article

   James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

   Insert

   butelase 1
   

   Use

   Tags

   6-His

   Expression

   Mutation

   Promoter

   T5

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSAC5.02

   Plasmid

   #16077

   Depositor

   Jochen Buck, Lonny Levin

   Article

   Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

   Insert

   soluble adenylyl cyclase (Adcy10 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pCVD438

   Plasmid

   #63759

   Purpose

   eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.

   Depositor

   James Kaper

   Article

   Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

   Insert

   eae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pT2iU6m5Hoxa13bE4PCh(#765)

   Plasmid

   #184102

   Purpose

   cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   5myc-ZfHoxa13b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes) + in vitro tra…

   Tags

   5xMyc

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi+SP6

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDT2

   Plasmid

   #199119

   Purpose

   circular transcription template

   Depositor

   Jeff Gelles

   Article

   Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

   Insert

   lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT22U6CatalE4PCh(#767)

   Plasmid

   #184078

   Purpose

   cyclofen-inducible Catalase activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Insert

   CatalDeltaC-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes) + in vitro tra…

   Tags

   Expression

   Mutation

   CatalDeltaC: deleted 516-527; ERT2: G400V, M543A,…

   Promoter

   Zf-Ubi+SP6

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT22i5uasH3En2SE4P3Che(#1212)

   Plasmid

   #184098

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Insert

   3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Expression

   Mutation

   chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…

   Promoter

   5xUAS

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT22i5uasm5En25EE4P3Che(#1213)

   Plasmid

   #184099

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Insert

   5myc-chkEn2(5E)-ERT2-5'fP2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

   Promoter

   5xUAS

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT22i5uasm5En2SE4P3Che(#1209)

    Plasmid

    #184097

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Insert

    5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…

    Promoter

    5xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT22cUm5En2SE4P3Che(#1189)

    Plasmid

    #184095

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Insert

    5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT22cUm5En2E4P3Che(#1188)

    Plasmid

    #184094

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Insert

    5myc-chkEn2-ERT2-5'fP2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT22i5uasm5En2E4P3Che(#1208)

    Plasmid

    #184096

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Insert

    5myc-chkEn2-ERT2-5'fP2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …

    Promoter

    5xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tFucci(CA)5

    Plasmid

    #153521

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(CA)5.
    

    Use

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Use

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMA2640

    Plasmid

    #25434

    Purpose

    Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    reverse tetracycline-regulated transactivator Advanced
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMARO

    Plasmid

    #178086

    Purpose

    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Insert

    TcR (tetA/tetR)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Tetracycline resistance cassette is flanked by Ec…

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY2F

    Plasmid

    #53285

    Purpose

    Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJO629

    Plasmid

    #104342

    Purpose

    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

    Insert

    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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