We narrowed to 3,788 results for: golden gate

1. pAIP4_1EDG

   Plasmid

   #234144

   Purpose

   Heterologous protein expression of GUNA_RUMCH in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1EDG
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. U4m

   Plasmid

   #121017

   Purpose

   MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   UTR1
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nlsSalicS1

   Plasmid

   #232190

   Purpose

   Novel FRET-based biosensor for salicylic acid

   Depositor

   Alexander Jones

   Article

   Tang et al Science. 2025 Oct 9;390(6769):188-194. d

   Insert

   nlsSalicS1
   

   Tags

   His-tag

   Expression

   Plant

   Promoter

   p16

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. nlsSalicS1-NR

   Plasmid

   #232192

   Purpose

   Negative control for nlsSalicS1

   Depositor

   Alexander Jones

   Article

   Tang et al Science. 2025 Oct 9;390(6769):188-194. d

   Insert

   nlsSalicS1-NR
   

   Tags

   His-tag and Myc

   Expression

   Plant

   Promoter

   P16

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAV10.F3

   Plasmid

   #63199

   Purpose

   BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV10.KN

   Plasmid

   #63209

   Purpose

   pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEN-X-hPGK-PuroR-Z

   Plasmid

   #218904

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   hPGK-PuroR
   

   Expression

   Mammalian

   Mutation

   246T>A and 398T>C

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

   Plasmid

   #195049

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Expression

   Bacterial

   Promoter

   pPGI

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV10.K5

    Plasmid

    #63210

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV10.K4

    Plasmid

    #63212

    Purpose

    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. (K) tNOS (S)_T3 (SBE120)

    Plasmid

    #195010

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (K) tNOS (S)
    

    Expression

    Bacterial

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. (H) tNOS (I) _T2 (SBE119)

    Plasmid

    #195006

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (H) tNOS (I)
    

    Expression

    Bacterial

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. (B) HYG marker(R) (SBE264)

    Plasmid

    #195013

    Purpose

    hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+HYG+tGPD
    

    Expression

    Bacterial

    Promoter

    pXYL

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. (E) tNOS (F) _T1 (SBE118)

    Plasmid

    #194997

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (E) tNOS (F)
    

    Expression

    Bacterial

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. (B) BLE marker(R) (SBE262)

    Plasmid

    #195012

    Purpose

    bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+BLE+tGPD
    

    Expression

    Bacterial

    Promoter

    pXYL

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV113.loxP

    Plasmid

    #63186

    Purpose

    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV115.loxP

    Plasmid

    #63187

    Purpose

    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV116.loxP

    Plasmid

    #63188

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. cBEST4

    Plasmid

    #234660

    Purpose

    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

    Depositor

    Mingzi M. Zhang

    Article

    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

    Inserts

    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P3 and tcp830

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge