We narrowed to 15,743 results for: pET

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pET28a_Pro-IL-36β

Plasmid

#214344

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36B (IL36B Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pETM30-ARC105

Plasmid

#133426

Purpose

Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Expression

Bacterial

Mutation

Contains amino acids 5-78 fused to C-terminal of …

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pETM30-C9ORF78_F8A

Plasmid

#193369

Purpose

Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

Depositor

Markus Wahl

Article

Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

Insert

C9ORF78_F8A (C9orf78 Human)

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

F8A, the mutation is located in the binding inter…

Promoter

T7

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Tags

None

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET28 sf:mid.11

Plasmid

#191921

Purpose

Encodes a GFP design, sf:mid.11

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.11

Tags

His Tag

Expression

Bacterial

Mutation

Q69M Y145F T167I L220V V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.7

Plasmid

#191933

Purpose

Encodes a GFP design, sf:mid.7

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.7

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145F V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.6

Plasmid

#191912

Purpose

Encodes a GFP design, acid.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.6

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72A Y145M T167V H181L

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:acid.3

Plasmid

#191926

Purpose

Encodes a GFP design, sf:acid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:acid.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69L S72A T108V Y145M V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.7

Plasmid

#191900

Purpose

Encodes a GFP design, mid.7

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.7

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145F V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delZn1

Plasmid

#173933

Purpose

Bacterial expression of PARP1 lacking Zn1 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-HD

Plasmid

#174809

Purpose

Bacterial expression of isolated PARP1 HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-HD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmN

Plasmid

#221348

Purpose

Express ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Rhodoplanes-bGSDM

Plasmid

#212129

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Rhodoplanes bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Bacteroidetes-bGSDM

Plasmid

#212124

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Bacteroidetes bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Runella-bGSDM

Plasmid

#212123

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Runella bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Vitiosangium-bGSDM

Plasmid

#212127

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Vitiosangium bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Gillisia-bGSDM

Plasmid

#212128

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Gillisia bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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