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Plasmid#71468PurposeEncodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 5 (SENP5 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a RegIIIg
Plasmid#64936Purposebacterial expression of processed mouse RegIIIgammaDepositorInsertRegIIIg (Reg3g Mouse)
UseTagsExpressionBacterialMutationprocessed formPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETCON-HtsptLB12
Plasmid#45120DepositorInsertHtsptLB12
UseTagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastMutationPromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET3a hRegIIIa
Plasmid#64937Purposebacterial expression of processed human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
UseTagsExpressionBacterialMutationprocessed form. mutations made in 5' end to …PromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a-SF-LMNC-TS
Plasmid#169374PurposeE. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertLMNA residues 31-542 (LMNA Human)
UseTagsSplit-FlAsH Tag and TwinStrep TagExpressionBacterialMutationdeleted amino acid 1-30 (unstructured head domain…PromoterNative pET3a promotorAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP7
Plasmid#71470PurposeEncodes human full length SENP7. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 7 (SENP7 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmRPS27a
Plasmid#69559PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertRPS27A (RPS27A Human, Mouse)
UseTagsExpressionBacterialMutationChanged Alanine 133 (human RPS27a) to Glycine (mo…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PL-452 C-YPet
Plasmid#19177DepositorInsertYPet
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET15b-At 14-3-3 omega
Plasmid#44930DepositorInsert14-3-3 omega (GRF2 Mustard Weed)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Sad1
Plasmid#90297Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - CPX
Plasmid#89620Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertCytosolic Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
PL-452 N-YPet
Plasmid#19172DepositorInsertYPet
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET19b-MED17
Plasmid#15412DepositorInsertMED17 (MED17 Human)
UseTagsHisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETcon(I-LtrI)
Plasmid#41523DepositorInsertI-LtrI
UseTags3x(G4S) Linker, G3S Linker, HA Tag, and Myc TagExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP
Plasmid#84524PurposeExpresses murine SRAP domain (250 aa) in bacteriaDepositorInsertSRAP domain (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationPromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RL018A
Plasmid#42137DepositorInsertmCerulean-mCherry
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-mCherry
Plasmid#66533PurposeExpresses mCherry under the control of Mutant T7 Promoter G6DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - G6Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet11a-ABLE
Plasmid#158627PurposeAmp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expressionDepositorInsertApixaban-Binding Helical Bundle
UseTags6x His-tag and TEV cleavage tagExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only