We narrowed to 6,573 results for: tac

1. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

   Plasmid

   #196976

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   GST and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGST-parallell2-GGA3(571-723)

   Plasmid

   #199150

   Purpose

   Expresses GST-GGA3(571-723) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Miller et al Nat Struct Biol. 2003 Aug;10(8):599-606.

   Insert

   GGA3(571-723) (GGA3 Human)
   

   Use

   Tags

   GST and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

   Plasmid

   #166433

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM2 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

   Plasmid

   #166434

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM5 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

   Plasmid

   #166508

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM1 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

   Plasmid

   #166510

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM3 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

   Plasmid

   #166511

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM4 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EGFP-RanGAP*

   Plasmid

   #175237

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX4T3-Stx3-L77A/L80A

   Plasmid

   #100273

   Purpose

   Expresses GST-Stx3-L77A/L80A in bacteria.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

   Insert

   Stx3-1-265-L77A/L80A (Stx3 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   No transmembrane domain; residues 4-264 present, …

   Promoter

   Tac

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T3-Stx3-E78A/E83A

    Plasmid

    #100272

    Purpose

    Expresses GST-Stx3-E78A/E83A in bacteria.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

    Insert

    Stx3-1-265-E78A/E83A (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain; residues 4-264 present, …

    Promoter

    Tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX4T3-Stx3-LE165/166AA

    Plasmid

    #100271

    Purpose

    Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

    Insert

    Stx3-1-265-LE165/166AA (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain; residues 4-264 present, …

    Promoter

    tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJB03

    Plasmid

    #111961

    Purpose

    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Inserts

    mEGFP
    eDHFR
    

    Use

    Tags

    DHFR

    Expression

    Bacterial

    Mutation

    eDHFR;residues 2-159 and enhanced GFP with monome…

    Promoter

    Tac

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GST-RP2-C86Y,P95L

    Plasmid

    #86074

    Purpose

    A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed cysteine-86 and proline-95 to tyrosine an…

    Promoter

    tac

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GST-peripherin-CTS

    Plasmid

    #86070

    Purpose

    A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Peripherin (prph2.S Frog)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 317-336 of peripherin

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GST-cystin-CTS

    Plasmid

    #86066

    Purpose

    A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Cystin (Cys1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 28-35

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX 4T-1/MKRN1

    Plasmid

    #78757

    Purpose

    To purify GST-tagged MKRN1 recombinant protein

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFM110

    Plasmid

    #40156

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, 7SE (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    7SE; base pairs 6-523 of par-2 were recoded to c…

    Promoter

    tac

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pFM107

    Plasmid

    #40153

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, 7SA (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    7SA; base pairs 6-523 of par-2 were recoded to c…

    Promoter

    tac

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFM103

    Plasmid

    #40149

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, wild-type (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Base pairs 6-523 of par-2 were recoded to create…

    Promoter

    tac

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pFM109

    Plasmid

    #40155

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, C56S (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C56S; base pairs 6-523 of par-2 were recoded to …

    Promoter

    tac

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only