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SIK3 gRNA (BRDN0001148836)

Plasmid

#75756

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SIK3 gRNA (BRDN0001149197)

Plasmid

#75757

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NADK2 gRNA (BRDN0001146214)

Plasmid

#78091

Purpose

3rd generation lentiviral gRNA plasmid targeting human NADK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-dKARD

Plasmid

#114053

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-dKARD (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-A2-77p-Venus

Plasmid

#64856

Purpose

Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

Depositor

Sriram Neelamegham

Article

Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

Insert

VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)

Tags

7X His and Venus

Expression

Bacterial

Promoter

T7 Promoter

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T2 gp78 G2BR mt / JM26

Plasmid

#13308

Depositor

Allan Weissman

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

cDNA corresponds to aa309-643 derived from human …

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag p38 alpha

Plasmid

#20786

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pTE4565

Plasmid

#88903

Purpose

Expresses inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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TRIB2 gRNA (BRDN0001148332)

Plasmid

#75604

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB2 (TRIB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRV-GN

Plasmid

#106373

Purpose

creating stable line with GFP and G418 resistance

Depositor

Tong-Chuan He

Article

Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

Insert

eGFP and geneticin/G418 resistance gene

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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SIK1 gRNA (BRDN0001487095)

Plasmid

#77893

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK1 (SIK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SIK1 gRNA (BRDN0001146021)

Plasmid

#77894

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK1 (SIK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK2 gRNA (BRDN0001146083)

Plasmid

#76910

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK2 (RIPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.2 (20-362)

Plasmid

#177845

Purpose

Bacterial Expression of SnRK2.2

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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SYK gRNA (BRDN0001146072)

Plasmid

#76777

Purpose

3rd generation lentiviral gRNA plasmid targeting human SYK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SYK (SYK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA1 gRNA (BRDN0001148481)

Plasmid

#75499

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA1 (RPS6KA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RFK gRNA (BRDN0001146181)

Plasmid

#76847

Purpose

3rd generation lentiviral gRNA plasmid targeting human RFK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RFK (RFK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RFK gRNA (BRDN0001162491)

Plasmid

#76850

Purpose

3rd generation lentiviral gRNA plasmid targeting human RFK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RFK (RFK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTBK2 gRNA (BRDN0001147885)

Plasmid

#77968

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTBK2 (TTBK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-FLIP pr4

Plasmid

#19129

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +10…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only