We narrowed to 19,677 results for: Cre

1. hyBE4max

   Plasmid

   #157942

   Purpose

   Mammalian expression of hyBE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   hyBE4max
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Plxna3-Fc-His

   Plasmid

   #72124

   Purpose

   Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxna3 (Plxna3 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. W118-1_hTGM5-Flag

   Plasmid

   #180410

   Purpose

   lentiviral transduction of human TGM5 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM5 (TGM5 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. aMHC-puro rex-neo

   Plasmid

   #21230

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_UdgX-EE-UdgX-nCas9-UdgX

   Plasmid

   #163566

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_APOBEC1-UdgX-NG-nCas9

   Plasmid

   #163533

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

   Plasmid

   #163543

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MYH11-gRNA1

   Plasmid

   #132587

   Purpose

   gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

   Depositor

   James Thomson

   Article

   Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

   Insert

   MYH11-gRNA1
   

   Expression

   Bacterial

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTT3 Unc5D-FLAG

   Plasmid

   #72197

   Purpose

   Expresses full-length Unc5d with a C-terminal FLAG tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5d (Unc5d Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. W118-1_hTGM6-Flag

    Plasmid

    #180411

    Purpose

    lentiviral transduction of human TGM6 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM6 (TGM6 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MoFGFR3b-SV40PA

    Plasmid

    #86493

    Purpose

    mamalian cell expression of FGFR3b

    Depositor

    David Ornitz

    Article

    Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

    Insert

    FgfrR3 IIIb 12F5 (Fgfr3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Moloney murine leukemia virus LTR

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMV361_MjTyrRS

    Plasmid

    #193250

    Purpose

    Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

    Depositor

    Peter Schultz, Jessica Seeliger

    Article

    Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

    Insert

    MjTyrRS
    

    Expression

    Bacterial

    Promoter

    hsp60

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.18 NR0B1-Luc

    Plasmid

    #100983

    Purpose

    NR0B1 luciferase reporter - firefly luciferase driven by NR0B1 promoter

    Depositor

    Lee Helman

    Article

    Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

    Insert

    NR0B1 (NR0B1 Human)
    

    Expression

    Mammalian

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-MHL: nsp16_SARS-CoV-2

    Plasmid

    #167850

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pmiCas9-msa

    Plasmid

    #164833

    Purpose

    miCas9-msa fusion protein expressing plasmid

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miCas9-msa
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    contains mSA sequence range is 5106-5447 and Brex…

    Promoter

    CMV

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102797

    Purpose

    Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    H231L

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. S1PR1-DuET

    Plasmid

    #213374

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    S1PR1 (S1PR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mito-mTurq2-Apollo-NADP+

    Plasmid

    #193303

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    mTurqoise2 and mitochondrial targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sema6a.a-AP-His

    Plasmid

    #72037

    Purpose

    Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6a.a (Sema6a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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