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pSPK1

Plasmid

#18876

Depositor

Jay Keasling

Article

Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

Insert

polyphosphate kinase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only
pCS2+ Sizzled

Plasmid

#16688

Depositor

Marc Kirschner

Article

Salic et al Development. 1997 Dec . 124(23):4739-48.

Insert

Sizzled (szl Frog)

Use

Xenopus expression

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCLNDX

Plasmid

#12342

Depositor

Inder Verma

Article

Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pET15-ZmPYK

Plasmid

#220230

Purpose

Inducible expression of Zymomonas mobilis pyruvate kinase

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

Plasmid

#199585

Purpose

Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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IRSp53/BAIAP2 scFv [L117/1]

Plasmid

#190506

Purpose

Mammalian Expression of IRSp53/BAIAP2 scFV. Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IRSp53/BAIAP2 (Homo sapiens) recombinant scFV (BAIAP2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Kir6.1 K+ channel scFv [N366/60]

Plasmid

#190547

Purpose

Mammalian Expression of Kir6.1 K+ channel scFV. Derived from hybridoma N366/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj8 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Beta4-spectrin scFv [N393/2]

Plasmid

#190550

Purpose

Mammalian Expression of Beta4-spectrin scFV. Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Beta4-spectrin (Homo sapiens) recombinant scFV (SPTBN4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv4.2 K+ channel [L28/4R]

Plasmid

#149453

Purpose

Mammalian Expression Plasmid of anti-Kv4.2 K+ channel (Rat). Derived from hybridoma L28/4.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv4.2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnd2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Snapin/SNAPAP [L8/15R]

Plasmid

#140071

Purpose

Mammalian Expression Plasmid of anti-Snapin/SNAPAP (Human). Derived from hybridoma L8/15.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Snapin/SNAPAP (Human) recombinant mouse monoclonal antibody (SNAPIN Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCK354

Plasmid

#129698

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120010799

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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K51/1RAnti-SH3GL1/Endophilin A2 [K51/1R]

Plasmid

#128621

Purpose

Mammalian Expression Plasmid of anti-SH3GL1/Endophilin A2 (Rat). Derived from hybridoma K51/1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SH3GL1/Endophilin A2 (Rat) recombinant mouse monoclonal antibody (Sh3gl1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Kvbeta K+ channel [K25/73R]

Plasmid

#128617

Purpose

Mammalian Expression Plasmid of anti-Pan-Kvbeta K+ channel. Derived from hybridoma K25/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Kvbeta K+ Channel Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEcRNAP5

Plasmid

#128939

Purpose

pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

Depositor

Seth Darst

Article

Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

Inserts

RpoAppx234-241-(His)10 (rpoA Escherichia coli)
RpoB
RpoC
RpoZ

Tags

His

Expression

Bacterial

Mutation

Residues 234-241 are substituted for a PreScissio…

Promoter

T7

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Flrt3 [N353/13R]

Plasmid

#114486

Purpose

Mammalian Expression Plasmid of anti-Flrt3 (Mouse). Derived from hybridoma N353/13.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Flrt3 (Mus musculus) recombinant mouse monoclonal antibody (Flrt3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Navbeta4 Na+ channel [N168/6.3R]

Plasmid

#114505

Purpose

Mammalian Expression Plasmid of anti-Navbeta4 Na+ channel (Rat). Derived from hybridoma N168/6.3.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Navbeta4 Na+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Scn4b Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-LRRK1 [N341/37R]

Plasmid

#114503

Purpose

Mammalian Expression Plasmid of anti-LRRK1 (Human). Derived from hybridoma N341/37.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-LRRK1 (Homo sapiens) recombinant mouse monoclonal antibody (LRRK1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-5 [N195A/16R]

Plasmid

#114481

Purpose

Mammalian Expression Plasmid of anti-QKI-5 (Human). Derived from hybridoma N195A/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-QKI-5 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-DCRD-YFP

Plasmid

#87450

Purpose

rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

DCRD (distal C-terminal regulatory domain) (CACNA1F Human)

Tags

6xHis-T7, FKBP, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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GST-GIV-CT

Plasmid

#69863

Purpose

Bacterial expression of GST tagged GIV-CT

Depositor

Pradipta Ghosh

Article

Garcia-Marcos et al J Biol Chem. 2010 Apr 23;285(17):12765-7

Insert

GIV/Girdin (CCDC88A Human)

Tags

GST

Expression

Bacterial

Mutation

C terminal amino acids 1623-1870 of human GIV

Promoter

TAC

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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