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Plasmid#202333PurposeDesign 98.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert98.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRER-PhoCl-Insert
Plasmid#219675PurposeCaging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2DepositorInsertRER-PhoCl
UseTagsPhoClExpressionMammalianMutationPromoterTREAvailable sinceJune 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTSAR3.1t
Plasmid#210254PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCerulean
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PADH1-TIR1-9myc-URA3
Plasmid#207016PurposeExpresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).DepositorInsertTIR1
UseTags9xMycExpressionYeastMutationPromoterpADH1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-Y387A-GFP
Plasmid#207022PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationY387APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A-GFP
Plasmid#207021PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationD384APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-I375A-GFP
Plasmid#207020PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationI375APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A-GFP
Plasmid#207019PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
UseTagsGFPExpressionYeastMutationW371APromoterpTEF1Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vph1-2GFP-URA
Plasmid#207009PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertVPH1
UseTags2xGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
UseTagsExpressionYeastMutationFour Arg to Ala mutations in zinc finger backbonePromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA335
Plasmid#216069PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; StopDepositorHas ServiceCloning Grade DNAInsertStop; IRES2_v1; Start; HygroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEdv1
Plasmid#218283PurposeUse together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITEu2
Plasmid#218282PurposeUse together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locusDepositorInsertHO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJE13HD7
Plasmid#218281PurposeUse together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.DepositorInsertHO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4562
Plasmid#200780PurposePflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFPDepositorInsertGtAR2
UseTagsmCherryExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only