We narrowed to 88,360 results for: MAL

1. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139087

   Purpose

   Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-ACTB_sgRNA

   Plasmid

   #183885

   Purpose

   pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB sgRNA spacer (ACTB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_hE2F7mg

   Plasmid

   #73073

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns

   Promoter

   CMV

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCGN K-Ras 12V, 188L

   Plasmid

   #14722

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Insert

   K-ras (KRAS Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   12V, 188L

   Available Since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pHR-ZIP(WT)

   Plasmid

   #27134

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQLL-Tc1-8B_DR[EF1α-copGFP]

   Plasmid

   #218327

   Purpose

   Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   copGFP mammalian expression cassette
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM60-FLAG-SLC38A9.1 (1-119aa)

   Plasmid

   #71862

   Purpose

   lentiviral stable expression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized, first 119 amino acids

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pTBL680 CHYRON3 integration construct

   Plasmid

   #126448

   Purpose

   To integrate the CHYRON3 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6/3xLacO-CHYRON3 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6/3xLacO

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.E2.ChETA

   Plasmid

   #220310

   Purpose

   Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

   Depositor

   Matt Rowan

   Article

   Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

   Insert

   ChETA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   S5E2

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

    Plasmid

    #71217

    Purpose

    dCas9 fused to human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B catalytic domain
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOS009: pLX304-QuasAr2

    Plasmid

    #62985

    Purpose

    Lentivirus production of QuasAr2 fluorescent reporter

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr2-mOrange
    

    Use

    Lentiviral; Gateway destination vector

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-MED4

    Plasmid

    #15425

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED4 (MED4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. Raichu-OsRac1 CA

    Plasmid

    #133264

    Purpose

    Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(G19V) (LOC4325879 Oryza sativa)
    

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 G19V

    Promoter

    Ubi

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV/myc/Nuc-mutant-hogg1

    Plasmid

    #18710

    Depositor

    David Sidransky

    Article

    Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

    Insert

    8-oxoguanine DNA glycosylase (OGG1 Human)
    

    Expression

    Mammalian

    Mutation

    Amino Acid 229 Arginine changed to Glutamine

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

17. FLAG-MED10

    Plasmid

    #15417

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED10 (Med10 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

    Plasmid

    #108833

    Purpose

    Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa28 to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS and GAI

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCCLc-U6-shHMGA2.3-PGK-dTomato

    Plasmid

    #89606

    Purpose

    Silencing Hmga2 in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    shRNA targeting HMGA2 (HMGA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

    Plasmid

    #163545

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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