We narrowed to 20,018 results for: Nov
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Plasmid#149589Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
ExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2B_Psyn-sgRNA500
Plasmid#149558PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_Psyn-sgRNAflaB
Plasmid#149560PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRExpressionBacterialPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_Psyn-sgRNAftsI
Plasmid#149562PurposeftsI gRNA expression in B. burgdorferiDepositorInsertsgRNAftsI
UseCRISPRExpressionBacterialPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAftsI
Plasmid#149563PurposeftsI gRNA expression in B. burgdorferiDepositorInsertsgRNAftsI
UseCRISPRExpressionBacterialPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_Psyn-sgRNAmreB
Plasmid#149566PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRExpressionBacterialPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAmreB
Plasmid#149567PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRExpressionBacterialPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_Psyn-sgRNArodA
Plasmid#149568PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRExpressionBacterialPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m8
Plasmid#84727PurposepVR2 but with two G to U mutations immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationG to U mutations at positions 40 and 41 of annota…Available sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…Available sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5 (R511C)-V5 blast
Plasmid#83104PurposeLentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Arginine 511 to CysteineAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG LacZ
Plasmid#83093PurposeGateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tagDepositorInsertLacZ
UseGateway CloningTags3xFLAGPromoterTRE tight promoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN_TT 3xFLAG SMAD4
Plasmid#83094PurposeGateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tagDepositorAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5 (T288P)-V5 blast
Plasmid#83101PurposeLentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Threonine 288 to ProlineAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304 PCSK5 (A270T)-V5 blast
Plasmid#83102PurposeLentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Alanine 270 to ThreonineAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5 (H516P)-V5 blast
Plasmid#83103PurposeLentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Histidine 516 to ProlineAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-BsCas12b
Plasmid#121952PurposeE. coli expression, protein purificationDepositorInsertBsCas12b
Tags3xHA tag and 10xHis tagExpressionBacterialAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
gamma2 UTR shRNA (shRNA4)
Plasmid#120796PurposeshRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNADepositorInsertGABRG2 shRNA (Gabrg2 Rat)
UseRNAiAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107321PurposeExpresses SpCas9 (R1333S) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1333S) is fused to SaCas9PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseExpressionBacterialPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only