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pcDNA5FRT/TO-FAF1-Strep-HA

Plasmid

#113486

Purpose

Expression of human FAF1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

FAF1 (FAF1 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NL-BCL2L1

Plasmid

#113458

Purpose

NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 like 1 (BCL2L1 Human)

Use

Luciferase

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR U6-hTERTsh

Plasmid

#111385

Purpose

Knock down of hTERT transcript

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

shRNA C3 against hTERT

Use

Gateway entry cloning

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLX303-PCDH7-d

Plasmid

#108541

Purpose

Expresses human PCDH7 transcript variant d in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant d (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBA1A

Plasmid

#111695

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBA1A (TUBA1A Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSC10

Plasmid

#104784

Purpose

pAH595-gRNA 2xplex entry cassette

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-HA-YWAA-pGK-HYG

Plasmid

#107379

Purpose

Lentiviral expression of HA-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR AA Clover

Plasmid

#90238

Purpose

Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AA mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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ADAMTS5_pCSdest

Plasmid

#53819

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADAMTS5 (ADAMTS5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSC5

Plasmid

#104779

Purpose

pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDR FLIP43 GPS1

Plasmid

#101084

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Tags

6HIS and cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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EMR2_pLX307

Plasmid

#98332

Purpose

Lentiviral expression of EMR2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

EMR2 (ADGRE2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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PPP6C_pLX307

Plasmid

#98360

Purpose

Lentiviral expression of PPP6C

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

PPP6C (PPP6C Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/RFP-DDX54

Plasmid

#97061

Purpose

Expresses RFP (tdTomato)-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

RFP (tdTomato)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302 LacZ-V5 puro

Plasmid

#98579

Purpose

Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pGem-sHyper:sAPX

Plasmid

#84738

Purpose

Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
sHyper (HyPer2 targeted to nucleus)

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Promoter

pFMV/p35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pUB-spHRed

Plasmid

#84734

Purpose

Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

pHRed

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDD372

Plasmid

#91824

Purpose

Codon-optimized GFP donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

GFP-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJG80

Plasmid

#89282

Purpose

Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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