We narrowed to 168,274 results for: addgene
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Plasmid#166716PurposeVector expressing AAVR (KIAA0319L) with a C-terminal Flag tagDepositorAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only
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pHIV-EGFP.Flag-EZH2
Plasmid#220243PurposeExpresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.DepositorAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP-LMNB1
Plasmid#207773PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cytosolic Fyn FRET Biosensor
Plasmid#124654PurposeFRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPetDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
ExpressionMammalianAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
[#GS16-LV] Syn1-MitoTRACER - Donor
Plasmid#233565PurposeLentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotorDepositorInsertMitoTRACER-Donor
UseLentiviral and Synthetic BiologyTagsGFP11 and HA TagExpressionMammalianAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
CDK2_HUMAN_D0
Plasmid#79726PurposeThis plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh and U6Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-tRNApromo-sgRNA-espCas91_1
Plasmid#167210PurposePlasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCMVAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1A_Hygro_P2A_RUNX1_R118G_Barcode
Plasmid#170970PurposeOverexpression of RUNX1 R118G mutant protein.DepositorAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1A_Hygro_P2A_RUNX1_G100V_Barcode
Plasmid#170955PurposeOverexpression of RUNX1 G100V mutant protein.DepositorAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-KRAB-dCas9-P2A-mCherry
Plasmid#60954Purpose2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherryDepositorInsertKRAB-dCas9-P2A-mCherry fusion
UseCRISPR and LentiviralTags2x NLS, HA, KRAB domain, and mCherryExpressionMammalianPromoterSFFVAvailable SinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKII-TRPV1-flag
Plasmid#238924PurposeExpression of the human TRPV1 channel with a Flag-tag in excitatory neuronsDepositorInsertCaMKII-TRPV1-flag
UseAAVTagsflagExpressionMammalianPromoterCaMKIIalfaAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP
Plasmid#64323PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-EBFP2ExpressionMammalianPromoterCBh and U6Available SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pABpuro-BluF
Plasmid#46824Purposeluciferase reporter for BmalI promoterDepositorInsertBmal1 (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianPromoterBmal1Available SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)
Plasmid#140002PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6f
Plasmid#128315PurposeExpresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorHas ServiceAAV1InsertGCaMP6f
UseAAVTagsHis TagExpressionMammalianPromoterEf1aAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001148862)
Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI
Plasmid#201683PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.DepositorInsertNme2-ABE8e-i1
UseAAV and CRISPRExpressionMammalianPromoterU1aAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_dC_mKate2
Plasmid#167336PurposeExpresses mKatet2 fused to TFAM dC-tail mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) as well a…PromoterCMVAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only