We narrowed to 14,168 results for: flag

1. VP12

   Plasmid

   #72247

   Purpose

   Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9 plasmid

   Plasmid

   #100091

   Purpose

   dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFlag-NLS-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CSII-IDR2-humanTRIM5-FOS

   Plasmid

   #79066

   Purpose

   Express human TRIM5alpha in mammalan cells

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   TRIM5alpha(human)-FOS (TRIM5 Human)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Egr1

   Plasmid

   #11729

   Depositor

   Eileen Adamson

   Article

   Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

   Insert

   Egr-1 (Egr1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-Hsp70-Cas9

   Plasmid

   #46294

   Purpose

   A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

   Depositor

   Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x Flag

   Expression

   Insect

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Hsp70

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IPU-GFP-MYH9

   Plasmid

   #168273

   Purpose

   Expresses MYH9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   MYH9 (MYH9 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. NPY5R-Tango

   Plasmid

   #66456

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   NPY5R (NPY5R Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. NPY2R-Tango

   Plasmid

   #66454

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   NPY2R (NPY2R Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGTQL1221YC

    Plasmid

    #61705

    Purpose

    Gateway compatible BiFC vector (pEarleyGate202-YC)

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Bifc assay

    Tags

    YFP-C and FLAG

    Expression

    Plant

    Promoter

    35S

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO mTRPM7

    Plasmid

    #45482

    Depositor

    Andrew Scharenberg

    Article

    Nadler et al Nature. 2001 May 31;411(6837):590-5.

    Insert

    TRPM7 (Trpm7 Mouse)
    

    Use

    Tetracycline inducible

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    Tetracycline-controlled CMV

    Available Since

    May 21, 2013

    Availability

    Academic Institutions and Nonprofits only

12. SSTR2-Tango

    Plasmid

    #66503

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SSTR2 (SSTR2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. TSHR-Tango

    Plasmid

    #66518

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TSHR (TSHR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. SynTagMA_post

    Plasmid

    #119736

    Purpose

    Synaptic Tag for Mapping Activity

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    hSyn-1

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MRGPRX2-Tango

    Plasmid

    #66440

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MRGPRX2 (MRGPRX2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

    Plasmid

    #162333

    Purpose

    Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSpCas9-2xVP64
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SSTR5-Tango

    Plasmid

    #66506

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SSTR5 (SSTR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pmiHiFiCas9

    Plasmid

    #164832

    Purpose

    miHiFiCas9 expressing plasmid

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miHiFiCas9 (contains Brex27 at sequence 5031-5138).
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    change R 691 to A

    Promoter

    CMV

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-BCL6-IRES-GFP

    Plasmid

    #31391

    Depositor

    Hilda Ye

    Article

    Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

    Insert

    B-cell lymphoma 6 (BCL6 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFETCh_KLF9

    Plasmid

    #86286

    Purpose

    Donor vector for 3' FLAG tag of human KLF9

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KLF9 homology arms (KLF9 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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