We narrowed to 24,431 results for: nes

1. pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP

   Plasmid

   #205704

   Purpose

   Gal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila flies

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola5-EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20 Gal4

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBpuro-UbC-4493NES(Booster-PKA)

   Plasmid

   #184711

   Purpose

   PKA biosensor

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   Booster-PKA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TT mCh-V5-NES-EGFP-mCBP80

   Plasmid

   #192313

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-mCBP80
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TT mCh-V5-NES-EGFP-IBB

   Plasmid

   #192314

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-IBB

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-IBB
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-myc-OGA(GS-544)C181_NES

   Plasmid

   #193998

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181_NES

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181_NES
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

   Plasmid

   #107303

   Purpose

   Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   ZFP_VEGFA-TS2
   

   Use

   CRISPR

   Tags

   2xNES, 3x Flag, 2xNLS, and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

   Plasmid

   #107304

   Purpose

   Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   ZFP_VEGFA-TS3
   

   Use

   CRISPR

   Tags

   2xNES, 3x Flag, 2xNLS, and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mCherry-PKCsub-RLuc8N-NES

   Plasmid

   #138213

   Purpose

   Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKCsub-RLuc8N-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

   Plasmid

   #138299

   Purpose

   Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKCsub(T/A)-RLuc8N-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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