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pSMT3-fecB

Plasmid

#240158

Purpose

expresses FecB of M. marinum in mycobacteria

Depositor

Wilbert Bitter

Article

Lissner et al mBio. 2025 Dec 12:e0211925. doi: 10.1128

Insert

fecB (MMAR_1650)

Expression

Bacterial

Promoter

HSP60

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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L3928 Filler DNA

Plasmid

#244193

Purpose

Empty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN1>Fas-Myc/Kir2.1*(E224G Y242F):P2A :dTomato

Plasmid

#242233

Purpose

Express Kir 2.1 and dTomato in the neurons

Depositor

Qingchun Tong

Article

Li et al Cell Rep. 2025 Jun 19;44(7):115863. doi:

Inserts

Fas
Myc/Kir2.1 (E224GY242F)

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

SYN

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.1

Plasmid

#235053

Purpose

Expresses Tad-CBE2.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE2.5

Plasmid

#235054

Purpose

Expresses Tad-CABE2.5 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE2.5-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.1

Plasmid

#235056

Purpose

Expresses Tad-CBE3.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.2

Plasmid

#235057

Purpose

Expresses Tad-CBE3.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.1

Plasmid

#235051

Purpose

Expresses Tad-CABE1.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.2

Plasmid

#235052

Purpose

Expresses Tad-CABE1.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SV40-puro-EF1a-HA-NLGN1dAChE-bc

Plasmid

#220437

Purpose

Expresses HA-tagged human NLGN1 with K578A/V579A substitutions

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA-tag

Expression

Mammalian

Mutation

K578A and V579A substitutions

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--Pconst-YFP

Plasmid

#228099

Purpose

recA promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--Pconst-YFP

Plasmid

#228100

Purpose

constitutive promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--PrecA-YFP

Plasmid

#228101

Purpose

constitutive promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--PgadX-YFP

Plasmid

#228102

Purpose

constitutive promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--PrecA-YFP

Plasmid

#228094

Purpose

gadX promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--PgadX-YFP

Plasmid

#228095

Purpose

gadX promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--Pconst-YFP

Plasmid

#228096

Purpose

gadX promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--PgadX-YFP

Plasmid

#228098

Purpose

recA promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGL2-deltaTAR-luciferase

Plasmid

#215896

Purpose

Expression of luciferase

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

luciferase

Use

Luciferase

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-CERS5

Plasmid

#166158

Purpose

Gateway entry vector for N. furzeri CERS5

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

CERS5

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-FL

Plasmid

#215839

Purpose

Expression of FLAG/HA tagged firefly luciferase (FL)

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA tagged firefly luciferase (FL)

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL661-WT(TTTT)

Plasmid

#215864

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL661 with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL69-WT(TTTT)

Plasmid

#215847

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL864R-WT(TTTT)

Plasmid

#215860

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL864R with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL661-TTTA

Plasmid

#215865

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL661 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-EGFP12-TTTA

Plasmid

#215857

Purpose

This plasmid encodes sgRNA that target EGFP with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-EGFP12 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL714-TTTA

Plasmid

#215867

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL714 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSuperCRISPR-sgRNA-RL69-ATTT

Plasmid

#215848

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with ATTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSuperCRISPR-sgRNA-RL714-WT(TTTT)

Plasmid

#215866

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL714 with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL69-CTTT

Plasmid

#215852

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with CTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge