We narrowed to 4,125 results for: Alk

1. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20944

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pDONR223_HcRed

   Plasmid

   #25892

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   hcRed
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEM246 - MCS mosTI unc-119 target

   Plasmid

   #159821

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI unc-119 target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-hIes6

   Plasmid

   #15356

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   hIes6 (INO80C Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

   Plasmid

   #182351

   Purpose

   Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

   Plasmid

   #182358

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Pmex-5

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AMPK(T>A)-SPARK

   Plasmid

   #226256

   Purpose

   phospho-null mutant of AMPK-SPARK

   Depositor

   Roland Malli

   Article

   Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

   Insert

   AMPK(T>A)-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   substituted Threonine 20 with Alanine

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH3626

   Plasmid

   #179630

   Purpose

   Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2(s)

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVRb20_992

   Plasmid

   #49714

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   sfgfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pecf20_992

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVRa20_992

    Plasmid

    #49673

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF20_992
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEW3: pET-32a Dot1L(2-416)

    Plasmid

    #124098

    Purpose

    Dot1L(2-416) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-416) (DOT1L Human)
    

    Use

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-TAT-1-101-FLAG basic mutant

    Plasmid

    #35985

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1, 101 aa; R to A mutations at aa 56,…

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet RagA(T21N)-RagC

    Plasmid

    #99644

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    T21N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVRb01_syn20

    Plasmid

    #49704

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    sfgfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pecf01_syn20

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAluYNF1

    Plasmid

    #50933

    Purpose

    Expresses dimeric AluY RNA in mammalian cells.

    Depositor

    Katharina Strub

    Article

    Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

    Insert

    AluYNF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7SL RNA promoter (pol III)

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEPQD2KN0908

    Plasmid

    #177088

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    P19, CrDXS2, PaGPPS1, and CrGES
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDuet RagA-RagC(D181N)

    Plasmid

    #99643

    Purpose

    Bacterial expression of RagA-RagC(D181N)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D181N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK 3xFLAG-TWIST2 neo

    Plasmid

    #197936

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVRc20_992

    Plasmid

    #49739

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS20_992
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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