We narrowed to 4,825 results for: Drosophila

Showing: 1021 - 1040 of 4825 results

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pUb dRxCas13d + RxCas13d guide RNA

Plasmid

#176304

Purpose

Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Publication

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

RxCas13d

Tags

HA tag

Expression

Insect

Mutation

R239A, H244A, R858A, H863A

Promoter

Ubi-p63e

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUb dPspCas13b + PspCas13b guide RNA

Plasmid

#176306

Purpose

Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Publication

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

PspCas13b

Tags

HA tag

Expression

Insect

Mutation

H133A, H1058A

Promoter

Ubi-p63e

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-FLPo-3xP3-RFP

Plasmid

#127561

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

FLPo

Use

CRISPR

Expression

Insect

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::DsRed, Pactin::Ptrsps}

Plasmid

#86862

Purpose

Expresses DsRed in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Expression

Insect

Mutation

S129A

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pRB20

Plasmid

#52559

Purpose

serves as PCR-template to generate HR donors for C-terminal YFP-tag

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

eYFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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p{CFD4-EYFP-3xP3::DsRed}

Plasmid

#86863

Purpose

Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T2A-Gal4

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

Plasmid

#62946

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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KRigor980

Plasmid

#129763

Purpose

Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

Depositor

William Hancock

Insert

KHC (Khc Fly)

Tags

His6

Expression

Bacterial

Mutation

R210A

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

Plasmid

#62953

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Publication

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJAT56

Plasmid

#204300

Purpose

Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3XP3-EGFP

Use

CRISPR

Promoter

3XP3

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBac{Mhc-453-GFP,attB,w+}

Plasmid

#182199

Purpose

EGFP driven by 453bp enhancer of Mhc gene

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRVV655

Plasmid

#119019

Purpose

Drives TP901-1 recombinase expression in the D. melanogaster germ line

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 recombinase

Tags

SV40 NLS

Expression

Insect

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV647

Plasmid

#87630

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bxb1 attP

Use

CRISPR

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRB32

Plasmid

#72863

Purpose

template for N-terminal 1x Strep tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

single Strep tag

Expression

Insect

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab18 DN

Plasmid

#53474

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab18 (Rab18 Fly)

Tags

YFP

Expression

Insect

Mutation

S19N

Promoter

GAL4

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab11 CA

Plasmid

#53472

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Tags

YFP

Expression

Insect

Mutation

Q70L

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab35 DN

Plasmid

#53495

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab35 (Rab35 Fly)

Tags

YFP

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab23 WT

Plasmid

#53484

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab23 (Rab23 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available since

May 12, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab7 CA

Plasmid

#37704

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only