We narrowed to 165,256 results for: addgene

1. AAV pCAG-FLEX-mRuby3-WPRE

   Plasmid

   #99279

   Purpose

   Can be used to generate AAV virus that will express mRuby3 in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mRuby3
   

   Use

   AAV and Cre/Lox

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe H-Ras

   Plasmid

   #18744

   Depositor

   Scott Lowe

   Insert

   H-Ras (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G12V

   Available Since

   July 18, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pTEAD1.1.0-gDNA

   Plasmid

   #112425

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

   Depositor

   Kevin White

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV Cbx1-V5

   Plasmid

   #175106

   Purpose

   Lentiviral expression of mouse Cbx1-V5

   Depositor

   Larry Gerace

   Insert

   Cbx1 (Cbx1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ARF1

   Plasmid

   #28168

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ARF1 (ARF1 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Available Since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCMV-T7-ABEmax-SpG

   Plasmid

   #195278

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

   Depositor

   Carsten Lederer, Petros Patsali

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AD-003

   Plasmid

   #25502

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   AD-003 (NTMT1 Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. 4QPP

   Plasmid

   #61964

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Mutation

   See comments

   Promoter

   Polyhedrin

   Available Since

   Sept. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pAS_MmaPylT_EF1_NES-MmaPylRS AF

   Plasmid

   #174902

   Purpose

   expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG and NES

   Expression

   Mammalian

   Mutation

   Y306A, Y384F

   Promoter

   EF1

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKD2 (PKD2A-c034)

    Plasmid

    #98226

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PKD2A (PKD2 Human)
    

    Use

    Baculovirus

    Tags

    TEV-His10-Flag

    Mutation

    pfam00520:Ion_trans 475-680

    Promoter

    polyhedrin

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER20-FLAG-hSRF (#799)

    Plasmid

    #78345

    Purpose

    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    TRE2

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

12. SUV39H1

    Plasmid

    #28113

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SUV39H1 (SUV39H1 Human)
    

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Mutation

    contains chromo domain only (amino acid residues …

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pELF2.1.0-gDNA

    Plasmid

    #132454

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ELF2 (ELF2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. P100 (3OMC)

    Plasmid

    #28159

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SND1 (SND1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Tudor domain (residues 650-910).

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

15. BRPF3

    Plasmid

    #28269

    Depositor

    Cheryl Arrowsmith

    Insert

    BRPF3 (BRPF3 Human)
    

    Tags

    His with TEV cleavage

    Expression

    Bacterial

    Mutation

    Contains PWWP domain (residues 1056-1195).

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. DTX3L

    Plasmid

    #28266

    Depositor

    Cheryl Arrowsmith

    Insert

    DTX3L (DTX3L Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains only carboxyl terminal domain (residues …

    Available Since

    April 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pTAL5-BB

    Plasmid

    #36033

    Depositor

    Tom Ellis

    Article

    Blount et al PLoS One. 2012;7(3):e33279. Epub 2012 Ma

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    PGAL1

    Available Since

    July 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5J3E (THYN1)

    Plasmid

    #79524

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    THYN1 (THYN1 Human)
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. PARP14 (PARP14A-c013)

    Plasmid

    #110254

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PARP14 (PARP14 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. RIPK2 (RIPK2A-c036)

    Plasmid

    #110268

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RIPK2 (RIPK2 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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