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Addgene

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We narrowed to 159,238 results for: addgene

Showing: 1021 - 1040 of 159238 results

  1. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid
    #58490
    Purpose
    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Glutamine
    Promoter
    Ubiquitin
    Available since
    Aug. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    58490-plasmid-map-sequence-id-90354

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  3. pUK21

    Plasmid
    #49788
    Purpose
    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)
    Depositor
    Joachim Messing
    Article
    Vieira et al Gene. 1991 Apr;100:189-94.
    Type
    Empty backbone
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lacZ
    Available since
    July 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49788-plasmid-map-sequence-id-160334

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  5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  9. pLKO-Tet-On-ARNTL-shRNA2

    Plasmid
    #166914
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166914-plasmid-map-sequence-id-331230

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  14. pAAV-CMV-FLEX-SaCas9-U6-sgTh

    Plasmid
    #159901
    Purpose
    Mutagenesis of Th
    Depositor
    Larry Zweifel
    Insert
    Th (Th Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159901-plasmid-map-sequence-id-317304

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  15. BMP2K

    Plasmid
    #73251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BMP2KA (BMP2K Human)
    Use
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Mutation
    pfam00069:Pkinase 52-311
    Promoter
    T7
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pXZ13lacZalpha

    Plasmid
    #160229
    Purpose
    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160229-plasmid-map-sequence-id-314764

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  19. pLKO-Tet-On-ARNTL-shRNA1

    Plasmid
    #166913
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166913-plasmid-map-sequence-id-425230

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  20. pAP1-3

    Plasmid
    #71258
    Purpose
    luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Insert
    3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71258-plasmid-map-sequence-id-131888

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Showing: 1021 - 1040 of 159238 results