We narrowed to 1,247 results for: membrane gfp

1. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-cGAS R71/75E C396/7A-HA

   Plasmid

   #130920

   Purpose

   Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   human cGAS R71/75E C396/7A
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 71 and 75 mutated to glutamate; aa 396 and 397…

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-TIRAP-cGASΔN-HA

   Plasmid

   #130921

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   TIRAP-ΔNcGAS
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the N ter…

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-Fyn-cGASΔN-HA

   Plasmid

   #130922

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Fyn-ΔNcGAS
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the dual …

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-FKBP12-C1

   Plasmid

   #67900

   Purpose

   Expresses a fusion of mCherry with FKBP12 in mammalian cells

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   mCherry-FKBP12
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-loxpSTOPloxp-lyn-NeonGreen

   Plasmid

   #175257

   Purpose

   membrane target protein labelling

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   Lyn membrane targeting motif
   

   Use

   Cre/Lox

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pCAG

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRB-mCherry-PLIN3

   Plasmid

   #222420

   Purpose

   To express a lipid droplet "anchor" for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   PLIN3 (PLIN3 Human)
   

   Tags

   FRB-mCherry

   Expression

   Mammalian

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK162_mCherry-4.1G-hardened

   Plasmid

   #46361

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   4.1G (EPB41L2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Modified for siRNA resistance to gcTccTcaTttCgaAc…

   Promoter

   CMV

   Available Since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTK38_mCherry-LGN-C

    Plasmid

    #46346

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    LGN C-terminal region (GPSM2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains aa365-684; deletion of S524

    Promoter

    CMV

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTK47_mCherry-LGN-hardened

    Plasmid

    #46347

    Purpose

    rescues the phenotypes caused by LGN RNAi

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    LGN (GPSM2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

    Promoter

    CMV

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTK158_mCherry-4.1G-deltaCTD-hardened

    Plasmid

    #46360

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G (EPB41L2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 886-1005, and modified for si…

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK157_mCherry-4.1G-CTD

    Plasmid

    #46359

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G C-terminal domain (EPB41L2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C-terminal domain from 886-1005 amino acids

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs-ERas CVLS-IP

    Plasmid

    #13828

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    ERas CVLS (Eras Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CSVA (224-227) changed to CVLS (CAAX sequence of …

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

15. iRFP-PAmCherry1-PH_PLCd1

    Plasmid

    #92167

    Purpose

    PI(4,5)P2 single molecule fluorescent reporter

    Depositor

    Xuelin Lou

    Article

    Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

    Insert

    iRFP-PAmCherry1-PH_PLCd1
    

    Tags

    iRFP-PAmCherry1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. iRFP-PAmCherry1-PH_GRP1

    Plasmid

    #92168

    Purpose

    PI(3,4,5)P3 single molecule fluorescent reporter

    Depositor

    Xuelin Lou

    Article

    Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

    Insert

    iRFP-PAmCherry1-PHgrp1
    

    Tags

    iRFP-PAmCherry1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p63(1-29)-mCherry-N1

    Plasmid

    #67895

    Purpose

    Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    p63RhoGEF(1-29)-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCherry-C1-DH

    Plasmid

    #67898

    Purpose

    Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-DH
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-C1-p63RhoGEF

    Plasmid

    #67896

    Purpose

    Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-p63RhoGEF
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYC3.60-C1

    Plasmid

    #67899

    Purpose

    Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    YC3.60
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only